LOCUS       sequence-278251-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2339
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccat gcctctgaaa gctcaatacc aggagatggt gaccttcgag
       61 gatgtggctg tgcacttcac caagacagaa tggactggcc tttctcctgc ccagagggcc
      121 ctgtacagaa gtgtgatgtt ggagaatttt gggaacctga ctgctttggg gtacccagtt
      181 cccaaacctg cactgatctc acttttggag agaggggaca tggcttgggg cctggaagca
      241 caggatgatc ccccagcaga gaggaccaaa aacgtctgta aagatgttga gaccaacatt
      301 gacagtgagt ccacattaat ccagggaatt tctgaagaaa gagatgggat gatgtcacat
      361 ggtcagctga agagtgtccc tcagagaact gacttcccag aaacacgtaa tgtggaaaag
      421 caccaggaca tccccacagt gaaaaatatc caaggaaagg ttccaagaat cccctgtgca
      481 aggaaacctt tcatatgtga ggagtgtggg aaatccttca gctacttttc ttactatgct
      541 agacaccaga gaatccacac tggggagaaa ccttttgagt gtagtgagtg tggaaaagcc
      601 tttaatggta attcttcgtt aattcggcac cagaggattc acactggaga gagaccctat
      661 cagtgtgagg agtgtgggcg agcctttaat gataatgcaa atctgatcag gcatcagaga
      721 atccacagtg gggacagacc ctattactgt acagagtgtg ggaatagttt cacgagtagt
      781 tccgagtttg ttatacatca gagaatccac actggggaga aaccctatga gtgtaatgag
      841 tgtggcaaag cttttgttgg taattcaccc ctacttcggc atcagaaaat ccacactgga
      901 gagaaaccct atgagtgtaa tgagtgtggc aaaagctttg gaaggacttc ccatctaagc
      961 caacatcagc gtattcacac aggggaaaag ccttattctt gtaaagtatg tggacaagcc
     1021 ttcaattttc atacaaaact aactcggcac cagagaattc acagtgagga gaaacccttt
     1081 gactgtgtag attgtggaaa agccttcagt gctcaggaac aattaaaaag gcatctgaga
     1141 attcatactc aggagtcttc ctatgtatgt gatgagtgtg gaaaagcctt gactagcaaa
     1201 agaaatcttc atcagcatca aagaatccat actggagaga aaccctatga gtgtagcaag
     1261 tatgagaagg cctttgggac ttcttcccag ctaggtcacc ttgagcatgt ctactctgga
     1321 gagaagcctg tgctggacat ttgtcgtttt ggcctcccag aattttttac ccccttttac
     1381 tggtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1441 tgtggaaagg acgacgtgag cttgacctta cgaggtct
//
