LOCUS       sequence-278254-        1661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2340
FEATURES             Location/Qualifiers
     source          1..1661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggacacct tgaaaatact tcagtggttt tcccacgagc tattttctct
       61 ctctgtgaaa aggaaaccag gaaattgact ctgtgcctgt tttcaagaag cagttccgcc
      121 gagcatcctg gggcctccaa gcctccgata agctcctcca gtatgacatc acgcatcttg
      181 ctacgccagc aactcatgcg tgagcagatg caggagcagg agcgcaggga gcagcagcag
      241 aagctgcagg cggcccagtt catgcaacag agagtgcccg tgagtcagac accagccata
      301 aacgtcagtg tgcccaccac ccttccctct gccacgcagg tgccgatgga agtccttaag
      361 gtgcagaccc acctcgaaaa ccccaccaag taccacatac agcaagccca acggcagcag
      421 gtaaagcagt acctttctac cactttagca aataaacatg ccaaccaagt cctgagcttg
      481 ccatgtccaa accagcctgg cgatcatgtc atgccaccgg tgccggggag cagcgcaccc
      541 aacagcccca tggctatgct tacgcttaac tccaactgtg aaaaagaggg attttataag
      601 tttgaagagc aaaacagggc agagagcgag tgcccaggca tgaacacaca ttcacgagcg
      661 tcctgtatgc agatggatga tgtaatcgat gacatcatta gcctagaatc aagttataat
      721 gaggaaatct tgggcttgat ggatcctgct ttgcaaatgg caaatacgtt gcctgtctcg
      781 ggaaacttga ttgatcttta tggaaaccaa ggtctgcccc caccaggcct caccatcagc
      841 aactcctgtc cagccaacct tcccaacata aaaagggagc tcacagagtc tgaagcaaga
      901 gcactggcca aagagaggca gaaaaaggac aatcacaacc tgattgaacg aagaagaaga
      961 tttaacataa atgaccgcat taaagaacta ggtactttga ttcccaagtc aaatgatcca
     1021 gacatgcgct ggaacaaggg aaccatctta aaagcatccg tggactatat ccgaaagttg
     1081 caacgagaac agcaacgcgc aaaagaactt gaaaaccgac agaagaaact ggagcacgcc
     1141 aaccggcatt tgttgctcag aatacaggaa cttgaaatgc aggctcgagc tcatggactt
     1201 tcccttattc catccacggg tctctgctct ccagatttgg tgaatcggat catcaagcaa
     1261 gaacccgttc ttgagaactg cagccaagac ctccttcagc atcatgcaga cctaacctgt
     1321 acaacaactc tcgatctcac ggatggcacc atcaccttca acaacaacct cggaactggg
     1381 actgaggcca accaagccta tagtgtcccc acaaaaatgg gatccaaact ggaagacatc
     1441 ctgatggacg acaccctttc tcccgtcggt gtcactgatc cactcctttc ctcagtgtcc
     1501 cccggagctt ccaaaacaag cagccggagg agcagtatga gcatggaaga gacggagcac
     1561 acttgttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1621 tcttgtggaa aggacgacgt tgccacaata ctatatcctt g
//
