LOCUS       sequence-27827-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mPA-GFP-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             630..662
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    676..692
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgatgaggc ggcaaagaac ttatcaagtg gtgtggtggt gggagacgga gctgtaggta
       61 aaacttgcct actgatcagt tacacaacca atgcatttcc tggagaatat atccctactg
      121 tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat ctgggcttat
      181 gggatacagc tggactagaa gattatgaca gattacgccc cctatcctat ccgcaaacag
      241 atgtgttctt aatttgcttt tcccttgtga gtcctgcatc attccaccac gtccgtgcaa
      301 agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta gtgggaacta
      361 aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag aagctgactc
      421 ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta aaatacctgg
      481 agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg atccgagcag
      541 tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg taaaagcttg
      601 cggccgcaca gctgtataca cgtgcaagcc agccagaact cgccccggaa gaccccgagg
      661 atctcgagtg tggaattgtg agcggataac aatttgtcga gggcccctgc aggtcaattc
      721 taccgggtag gtgagcgctt ttcccacgca gtctggag
//
