LOCUS       sequence-278274-       12349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2358
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8206..8794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8259..8279)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8677..8688)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8866..9099
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9177..9298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9214..9233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9268..9287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9338..9473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9493..9512)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9494..9512)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9522..9539)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9522..9538)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9531..9553)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9680..10135
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9767..9786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9977..9998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10161..10265
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10266..10334,10335..11126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10484..10503)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11297..11885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11786..11805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12039..12056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12173..12194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12209..12214,12215..12232,12233..12239)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12247..12263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12252..12274
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12271..12287
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12271..12287
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12306..12326
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12308..12326
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgaga tcgttcttgc agcaagatgt
     4141 taataagaca aaatctagac taaatgtgtt aaatgggctt gccaacaata tggatgattt
     4201 gaagataaac accgatatta ctggtgctaa agaagaactc ctagatgaca acaattttat
     4261 ctcagacaaa gagagcggag ttcataagcc aaaagattgt caaacatcat ttcagaaaaa
     4321 taatacgttg actctgcctg aagaactgtc aaaggacaaa tctgaaaacg ccttaagtgg
     4381 aggccagtct agtctattta tacatgctgg tgctcctact gtttctagtg aaaactttat
     4441 cttgcctaaa ggagctgctg ttaatggacc agtttcacac tcctccttaa ctaagacttc
     4501 caatatgaat aaaggcagtg tttcattaac cactggacag cctgtggatc agccaacaac
     4561 agaatcttgt tcaactttga aggtagcagc tgatcttcag ctgtctacac cacagaaagc
     4621 aagtcaacac caagttttat ttttgttatc agatgtagca catgctaaga atcccaccca
     4681 ttccaataaa aaactaccta cctctgcttc agttggttgt gacattcaga attcagtagg
     4741 gagtaatata aagtcagatg gcactttaat aaatcaagta gaggtgggtg aggatggtga
     4801 agatttattg gtgaaagatg attgtgtcaa tacagtaacg ggaatttcct caggtacaga
     4861 tggatttagg tcagaaaatg atacaaactg ggatccccaa aaagagttca ttcaatttct
     4921 tatgactaat gaggaaacag tagataaagc tccacctcat tctaaaatag gtctagaaaa
     4981 aaaaagaaag cgaaaaatgg atgtaagcaa gataactcgt tataccgagg attgctttag
     5041 tgattctaat tgtgtaccca ataaatcaaa aatgcaagaa gtagactttc tagaacaaaa
     5101 tgaagagcta caagcagtag actcacagaa atatgcatta tcaaaagtga agcctgaatc
     5161 aactgatgaa gacttagaat ctgtggatgc cttccaacat ctaatttata acccagataa
     5221 gtgtggagaa gagagttcac ctgttcatac tagcactttt ctttcaaata ccttaaaaaa
     5281 gaaatgtgaa gagagtgatt ctgagtcacc tgctactttc agtaccgaag agccatcatt
     5341 ctacccctgt acaaagtgca atgtgaattt tagggagaag aagcacctcc acaggcatat
     5401 gatgtatcat ttagatggga atagtcactt tcgccatctt aatgtcccaa ggccatatgc
     5461 ttgtagagaa tgtggacgga catttcgaga tcgcaattca cttctaaaac atatgattat
     5521 tcaccaggag agaagacaga agttgatgga ggaaattcgt gaattgaaag aacttcagga
     5581 tgaaggaaga agtgcacgat tacagtgtcc tcagtgtgtg tttggtacca attgccctaa
     5641 aacatttgtg caacatgcta aaacccatga aaaagataaa aggtactact gctgtgaaga
     5701 gtgtaacttc atggcagtga cagaaaatga attggaatgc catcgaggca ttgcacatgg
     5761 ggcagtggta aaatgcccta tggtcacttc tgatattgcc cagagaaaaa cacaaaaaaa
     5821 gactttcatg aaagactctg tagtaggatc atccaaaaaa tcagctacct acatatgtaa
     5881 gatgtgtcct tttactactt cagccaaaag tgttttaaaa aagcacacgg agtacttgca
     5941 ttcatcatca tgtgttgatt catttggtag tcctcttgga cttgataaaa gaaaaaatga
     6001 catccttgaa gaacctgtag atagtgatag cactaaaaca ttaactaaac aacagtcaac
     6061 cacatttcca aagaactctg ctttaaaaca agatgtgaag cgaacatttg gatcaacctc
     6121 acaatcaagt agtttttcaa aaattcataa gcggccacac agaatacaga aagctcggaa
     6181 aagcattgcc caatcaggtg taaacatgtg caatcaaaac agctctcctc ataagaatgt
     6241 tacaattaaa agcagcgttg accaaaaacc taagtatttc catcaagcag caaaagaaaa
     6301 gtctaatgcc aaggcaaata gccactattt gtatagacac aaatatgaaa actataggat
     6361 gatcaaaaaa tcaggtgaat catatcctgt gcatttcaaa aaagaagaag ctagttcatt
     6421 aaattcttta cacctgtttt catcatcaag taattctcac aacaatttta tttcagaccc
     6481 tcataagcct gacgccaaaa ggcctgaaag cttcaaagat cacagacgtg tagctgtaaa
     6541 gagagtaatt aaggaatcta agaaggaaag ttctgttgga ggggaagact tggatagcta
     6601 tccagatttt ttgcataaaa tgactgttgt cgttttgcaa aaacttaatt ctgctgaaaa
     6661 gaaagatagt tatgaaacag aagatgaaag ttcctgggat aatgttgagt taggagacta
     6721 cactacacag gccatagaag atgaaaccta tagtgatatt aatcaagagc atgtaaattt
     6781 attcccttta tttaagagca aagtggaagg tcaggagcct ggagaaaatg ctactcttag
     6841 ttatgaccaa aacgatggct tttattttga atactatgaa gatactggaa gtaacaactt
     6901 tttgcatgag atacatgatc ctcagcattt agaaactgca gatgcttcat tgtcaaagca
     6961 tagttctgtt tttcattgga ctgatttgtc tcttgagaag aaatcgtgtc cttactgccc
     7021 agcaacattt gaaacaggtg ttgggttatc aaatcatgtc agggggcatc ttcacagagc
     7081 aggattaagc tatgaagccc gtcatgttgt atcaccagaa caaatagcca caagtgacaa
     7141 aatgcagcat ttcaaaagaa ctggcacagg aacacctgtt aaacgagtta gaaaagctat
     7201 agagaagtct gaaaccactt ctgaacacac ttgtcagctc tgtggtggtt ggtttgatac
     7261 taaaattgga ttatcaaatc atgttagagg ccacttgaaa agacttggaa agacgaaatg
     7321 ggatgctcac aaatctccaa tctgtgttct gaatgagatg atgcaaaatg aagaaaaata
     7381 tgaaaaaatc ttaaaggcat tgaacagtcg tcgtattatt cccagaccat ttgtagctca
     7441 aaaacttgca tcaagtgatg actttatatc tcaaaatgtt atacctcttg aagcataccg
     7501 taatggccta aagactgaag ctctgtcagt gtctgcatca gaagaagaag ggctgaattt
     7561 cttaaatgaa tatgatgaaa caaaaccaga actgcccagt gggaaaaaga atcagtctct
     7621 tacactcata gaacttctta aaaataaaag gatgggagaa gaaaggaatt ctgctatttc
     7681 tcctcaaaag atccataatc agacagcaag aaagagattc gttcagaaat gcgttcttcc
     7741 attaaatgag gatagtccgt tgatgtatca gccacaaaaa atggacttga ctatgcactc
     7801 agccttagat tgtaagcaaa agaaatcaag gtcaagatct ggaagcaaga agaaaatgct
     7861 aacattacct catggtgctg acgaggttta cattctccga tgcaggtttt gtggcctagt
     7921 ctttcgagga cccttgtctg ttcaggaaga ctggattaag cacttacaac gacatattgt
     7981 aaacgctaat cttccacgga ctggagctgg catggtggaa gtcacgtcac tacttaaaaa
     8041 gcctgcctcc attacagaaa cttcattttc tctactaatg gccgaagcag cttcatagta
     8101 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     8161 ggacgagatc gaacacagac gtccacgctt acgcgttaag tcgacaatca acctctggat
     8221 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8281 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8341 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8401 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8461 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8521 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8581 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8641 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8701 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8761 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     8821 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8881 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8941 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9001 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9061 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     9121 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9301 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9421 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9481 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9541 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9601 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9661 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9721 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9781 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9841 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9901 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9961 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10021 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10081 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    10141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10261 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10321 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10381 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10441 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10501 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10561 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10621 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10681 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10741 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10801 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10861 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10921 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10981 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11041 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11101 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11161 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11221 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11941 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    12001 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    12061 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    12121 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12181 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12241 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12301 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
