LOCUS       sequence-27828-e         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mPA-GFP-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(22..40,41..63,64..82,83..105,106..124,125..147,
                     148..166,167..189,190..208,209..231,232..250,251..273,
                     274..292)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        325..363
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     360..384
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tttcgtcgtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
       61 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
      121 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
      181 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      241 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      301 agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt gggaggccta tataagcaga
      361 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      421 agaagacacc gggaccgatc cagcctccgc ggccccagat ccgtaaagtg aattcggagc
      481 atataccatg gcacaccat
//
