LOCUS       sequence-27829-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mVenus tagged LOV2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mVenus-LOV
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     217..238
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(287..304)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(414..438)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(435..473)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    548..566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    632..650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
       61 agcgggcgaa gcactgcagg ccgtagccga gggtggtcac gagggtgggc cagggcacgg
      121 gcagcttgcc ggtggtgctg atgaacttca gggtcagctt gccgtaggtg gcatcgccct
      181 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
      241 tgggcaccac cccggtgaac agctcctcgc ccttgctcac agaaccgtga tggtggtgat
      301 ggtgtgccat ggtatatgct ccgaattcac tttacggatc tggggccgcg gaggctggat
      361 cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga
      421 cggttcacta aacgagctct gcttatatag gcctcccacc gtacacgcct acctcgaccc
      481 gggtaccgag ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
      541 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
      601 gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact
      661 cgactttcac tttttntcta tcactgatag gg
//
