LOCUS       sequence-27830-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of mVenus tagged LOV2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mVenus-LOV
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..471
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     CDS             560..592
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    606..622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(690..713)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctcggnntgg angagctgta caagggttct ggaagtggat ctttggctac tacacttgaa
       61 cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc
      121 gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac
      181 tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc
      241 atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag
      301 ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt
      361 attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc
      421 atgctgatta agaaaactgc agaaaatatt gatgaggcgg caaaagaact tggatccgaa
      481 ttctcagatc tcggcgcgcc tgcaggtcga cggtaccggt tcgaagcttg cggccgcaca
      541 gctgtataca cgtgcaagcc agccagaact cgccccggaa gaccccgagg atctcgagtg
      601 tggaattgtg agcggataac aatttgtcga gggcccctgc aggtcaattc taccgggtag
      661 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      721 ggcngctaca caagtgg
//
