LOCUS       sequence-278305-        1826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2370
FEATURES             Location/Qualifiers
     source          1..1826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcacgac tgggctggag agtctgagtc tgggggaccg cggagctgcc
       61 cccaccgtca cctctagtga gcgcctagtc ccagacccgc cgaatgacct ccgcaaagaa
      121 gatgttgcta tggaattgga aagagtggga gaagatgagg aacaaatgat gataaaaaga
      181 agcagtgaat gtaatccctt gctacaagaa cccatcgctt ctgctcagtt tggtgctact
      241 gcaggaacag aatgccgtaa gtctgtccca tgtggatggg aaagagttgt gaagcaaagg
      301 ttatttggga agacagcagg aagatttgat gtgtacttta tcagcccaca aggactgaag
      361 ttcagatcca aaagttcact tgctaattat cttcacaaaa atggagagac ttctcttaag
      421 ccagaagatt ttgattttac tgtactttct aaaaggggta tcaagtcaag atataaagac
      481 tgcagcatgg cagccctgac atcccatcta caaaaccaaa gtaacaattc aaactggaac
      541 ctcaggaccc gaagcaagtg caaaaaggat gtgtttatgc cgccaagtag tagttcagag
      601 ttgcaggaga gcagaggact ctctaacttt acttccactc atttgctttt gaaagaagat
      661 gagggtgttg atgatgttaa cttcagaaag gttagaaagc ccaaaggaaa ggtgactatt
      721 ttgaaaggaa tcccaattaa gaaaactaaa aaaggatgta ggaagagctg ttcaggtttt
      781 gttcaaagtg atagcaaaag agaatctgtg tgtaataaag cagatgctga aagtgaacct
      841 gttgcacaaa aaagtcagct tgatagaact gtctgcattt ctgatgctgg agcatgtggt
      901 gagaccctca gtgtgaccag tgaagaaaac agccttgtaa aaaaaaaaga aagatcattg
      961 agttcaggat caaatttttg ttctgaacaa aaaacttctg gcatcataaa caaattttgt
     1021 tcagccaaag actcagaaca caacgagaag tatgaggata cctttttaga atctgaagaa
     1081 atcggaacaa aagtagaagt tgtggaaagg aaagaacatt tgcatactga cattttaaaa
     1141 cgtggctctg aaatggacaa caactgctca ccaaccagga aagacttcac tgaagatacc
     1201 atcccacgaa cacagataga aagaaggaaa acaagcctgt atttttccag caaatataac
     1261 aaagaagctc ttagcccccc acgacgtaaa gcctttaaga aatggacacc tcctcggtca
     1321 ccttttaatc tcgttcaaga aacacttttt catgatccat ggaagcttct catcgctact
     1381 atatttctca atcggacctc aggcaaaatg gcaatacctg tgctttggaa gtttctggag
     1441 aagtatcctt cagctgaggt agcaagaacc gcagactgga gagatgtgtc agaacttctt
     1501 aaacctcttg gtctctacga tcttcgggca aaaaccattg tcaagttctc agatgaatac
     1561 ctgacaaagc agtggaagta tccaattgag cttcatggga ttggtaaata tggcaacgac
     1621 tcttaccgaa ttttttgtgt caatgagtgg aagcaggtgc accctgaaga ccacaaatta
     1681 aataaatatc atgactggct ttgggaaaat catgaaaaat taagtctatc ttagtaatga
     1741 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1801 gaaccggaaa tgcctaacag aactct
//
