LOCUS       sequence-27831-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             628..660
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    674..690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgatgaggc ggcaaagaac ttatcaagtg tgnggtggtg ggagacggag ctgtaggtan
       61 ccctttagan tgatcagtta cacaaccaat gcatttcctg gagaatatat ccctactgtc
      121 tttgacaatt attctgccaa tgttatggta gatggaaaac cggtgaatct gggcttatgg
      181 gatacagctg gactagaaga ttatgacaga ttacgccccc tatcctatcc gcaaacagat
      241 gtgttcttaa tttgcttttc ccttgtgagt cctgcatcat tccaccacgt ccgtgcaaag
      301 tggtatcctg aggtgcggca ccactgtccc aacactccca tcatcctagt gggaactaaa
      361 cttgatctta gggatgataa agacacgatc gagaaactga aggagaagaa gctgactccc
      421 atcacctatc cgcagggtct agccatggct aaggagattg gtgctgtaaa atacctggag
      481 tgctcggcgc tcacacatcg aggcctcaag acagtgtttg acgaagcgat ccgagcagtc
      541 ctctgcccgc ctcccgtgaa gaagaggaag agaaaatgcc tgctgttgta aaagcttgcg
      601 gccgcacagc tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat
      661 ctcgagtgtg gaattgtgag cggataacaa tttgtcgagg gcccctgcag gngcaattct
      721 accggggtag gggaggcgct tttcccaggc agtctggagc atgc
//
