LOCUS       sequence-278311-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2389
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaagcct cagagggaat actgctcagg gtcctacaaa tgaagaagac
       61 tataaaaacg aaggccaatt atcaaggcaa acaaaatgtc ctgcacagaa gaaatcctct
      121 tttgagaaca cagtggtcag aaaagtgtca gtgacactca aagaaatttt cacaggggag
      181 gaaggccctg aatccagtga atttagtcta agcccaaacc ttgacgcaca acagaaaatt
      241 ccaaagggac atggatcccc aatatctagg aaaaactcca aagataattc agacttaatt
      301 aaacaccaaa gacttttctc acaaagaaaa ccttgtaaat gcaatgaatg tgaaaaagcc
      361 tttagttacc aatcagacct tcttgtacac agtagaattc atggtggaga aaagcctttt
      421 gaatgcaaca aatgtgggaa atctttcagc cgaagtacac accttattga acatcaaaga
      481 actcacactg gagagaaacc ttatgaatgc aatgaatgtg gaaaagcttt tagccggagc
      541 acacatctta gtctacatca gagaatccat actggagaaa aaccatatga atgtagtgaa
      601 tgtggaaaag cctttagccg aagcactaac cttagtcagc atcagcgaac tcatactcaa
      661 gaaaggcctt acaaatgtaa tgaatgtggg aaagccttcg gtgaccgttc aaccataatt
      721 cagcatcaac gaatacacac tggagagaat ccctatgaat gcagtaaatg tggaaaagct
      781 ttcagttgga tctcatcgct tactgaacat cagagaacac acactgggga gaacccctat
      841 gagtgcagtg aatgtgggaa agtgttcagt cgaagctcgt ctcttacaga acatcagaga
      901 atccacagtg gagaaaagcc tcacgagtgt agagtgtgtg gaaagggctt cagtcgaagc
      961 tcatccctta ttattcatca gagaactcat accggggaga agccgtacaa atgtaatgac
     1021 tgtggaaaag ccttctgtca gagttcaact ctgatcagac atcagcacct tcatactaaa
     1081 gagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1141 tgtggaaagg acgacgcttg aagttcttcg tgctctcc
//
