LOCUS       sequence-278317-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2391
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccatga gttggtgatg ttcagagatg tggctataga tgtctctcag
       61 gaggaatggg aatgcctgaa cccagcgcag aggaatttgt acaaggaggt gatgttggag
      121 aattatagca acttggtgtc acttggactt tctgtttcta agccagccgt aatctcctca
      181 ttggagcaag ggaaggagcc ctggatggtt gtgagggaag agacaggaag atggtgccca
      241 gacttggcat ccagagatga gccccagaag ttatctccaa aaagggatat ttatgaaaca
      301 gaattatccc agtgggttaa catggaagaa tttaaaagcc atagtcctga gagatcaatt
      361 ttcagcgcta tctgggaagg caactgtcat tttgagcaac atcagggaca agaggagggt
      421 tattttagac aacttatgat taaccatgaa aacatgccca tttttagcca acatacttta
      481 ctcactcaag aattttatga tagagagaaa atctctgaat gtaaaaagtg tagaaaaatc
      541 ttcagttacc atttattttt tagtcaccac aaaagaactc attctaaaga actttctgaa
      601 tgtaaagaat gcacagaaat tgttaataca ccatgccttt ttaaacaaca gacaattcaa
      661 aatggtgaca aatgcaatga gtgtaaagaa tgttggaagg cctttgttca ttgctcacaa
      721 cttaaacatc taagaattca taatggtgaa aaacgctatg aatgtaacga atgtgggaag
      781 gcctttaatt atggctcaga acttactcta catcaaagaa ttcacactgg tgagaaacct
      841 tatgaatgta aagaatgtgg gaaggccttt agacagcgat cacagcttac tcaacatcag
      901 agacttcata ctggtgaaaa accctatgaa tgtaagcaat gtgggaaggc ttttattcgt
      961 ggctttcaac ttactgaaca cctgcgactc catactggag agaaacctta tgaatgtaaa
     1021 gaatgtggaa agacttttag gcatcgctca catcttacta tacatcagag aattcatact
     1081 ggtgagaaac cctatgaatg tcgggaatgt gggaaggcct ttagctatca ctcaagcttc
     1141 tcacaccatc agaaaattca ttctggcaag aaaccttatg aatgtcatga atgtgggaag
     1201 gctttttgtg atggcttaca actaacccta catcagagga ttcatactgg tgagaaaccc
     1261 tatgagtgta aggaatgtgg gaagactttt agacagtgtt cacacctcaa aagacatcag
     1321 agaattcata ctggtgagaa acctcatgaa tgcatgatat gtggtaaggc ctttagactt
     1381 cattcacacc ttattcaaca tcaaagaatt catactggtg agaaacccta tgaatgtaag
     1441 gaatgtggga aggcctttag ctatcattca agcttctcac accatcagag aattcattct
     1501 ggaaagaaac cttatcaatg cgggaaggcg tttaatcata gattacaact taacttacat
     1561 cagactcttc atactggcga gaagccagtc aggtttcctc tcctccctcc ccatcctagt
     1621 ctagcatcat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1681 atatcttgtg gaaaggacga acaggcatct ggaaagcggc tctg
//
