LOCUS       sequence-278332-        1706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2397
FEATURES             Location/Qualifiers
     source          1..1706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtgga atctggagtg atttcaaccc tgatacctca ggatcctccg
       61 gaacaagaac taatactagt gaaagtagaa gataactttt cctgggatga gaaatttaag
      121 cagaatggga gtactcaatc ctgccaagaa ttgtttcgtc agcaattcag aaaattttgc
      181 taccaggaga cacctgggcc ccgggaggct ctgagccgac tccaggaact ttgctatcag
      241 tggctaatgc cagagttgca cacaaaggag cagatcttag aactgctggt actggagcag
      301 ttcctgagca ttctgcctga ggagctgcag atctgggttc agcaacataa tccagaaagc
      361 ggcgaggaag ctgtgaccct gttggaggat ttagagaggg agtttgatga cccagggcag
      421 caggtcccag ctagtccaca gggaccagca gtgccatgga aggatttaac atgtctcaga
      481 gcatcccaag agtcaacaga catccacctc cagcccttaa agacacagct gaaatcctgg
      541 aaaccatgcc tttcccctaa aagtgattgt gagaacagtg aaacagcaac aaaagagggc
      601 atctcagaag aaaaatcaca gggactccct caggaacctt catttcgagg aattagtgag
      661 catgaaagca atttagtgtg gaagcaagga agtgctacag gggagaaact aagatctcct
      721 tcccaagggg gcagttttag tcaagtgatc ttcacaaaca aatctctagg aaagagagac
      781 ctttatgatg aggctgaaag atgcttgatt ctaactacag actctataat gtgtcagaaa
      841 gttcctccag aagagagacc ttatagatgt gatgtatgtg ggcacagctt caagcagcat
      901 tcctctctaa cacaacatca gagaatccat actggagaaa agccctataa atgtaaccag
      961 tgtgggaagg cctttagttt gaggtcctat cttattattc atcagagaat tcatagtggt
     1021 gagaaagcat atgaatgtag tgaatgtggg aaagctttca atcagagctc agccctcatt
     1081 agacatcgga aaatccatac tggtgagaaa gcttgtaaat gtaatgagtg tggcaaagca
     1141 ttcagtcaaa gttcatatct cattatacat caaagaattc acactggtga gaaaccttat
     1201 gagtgtaatg aatgtgggaa aacctttagc cagagctcaa aactcattag acatcagcga
     1261 attcacacag gagagagacc ctatgaatgt aatgaatgtg gaaaagcttt caggcagagc
     1321 tcagagctga ttactcatca gagaatacat agtggagaga aaccctatga atgtagtgaa
     1381 tgtggaaaag ctttcagttt gagctcaaac cttatcagac atcagagaat tcatagtggg
     1441 gaggaacctt atcagtgtaa tgaatgtggc aaaactttca aaaggagctc agcccttgtt
     1501 cagcatcaga gaattcattc tggggatgaa gcttatatat gtaatgaatg tgggaaggct
     1561 ttcaggcaca gatcggtcct tatgcgccat caaagagtcc acactataaa gtagtaatga
     1621 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1681 gactagtgct agtgtatacc gtgctc
//
