LOCUS       sequence-278359-        2945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2416
FEATURES             Location/Qualifiers
     source          1..2945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccaccag ggtccggaca gcttctattt gggtcccacc tctccaagaa
       61 cgaaacagtt catgggatag gatcagaaag ctccaaggtc aggaatccat cttgggccaa
      121 gggactcctg gtctgcaacc tctccctgga acacccaggc agaagcagaa gagtcgcaga
      181 atagagaaag tcctagagtg gctgtttatt tcccaagagc agccaaaaat caccaagtcc
      241 tggggacctt tgtcattcat ggatgtgttt gtggatttta cctgggagga gtggcagctg
      301 ctagacccag cacagaagtg cctgtacagg agtgtgatgt tggagaacta tagcaacctg
      361 gtgtccctag ggtaccaaca caccaaacct gatatcatct tcaagttgga acaaggagaa
      421 gagctgtgta tggtgcaggc ccaagttcca aatcagacct gtccaaacac agtctggaaa
      481 attgatgatc ttatggattg gcatcaggaa aataaagaca agctgggaag tacggcaaaa
      541 agctttgaat gcactacatt tggaaaacta tgtcttctta gtacaaagta tctttcaaga
      601 caaaaacctc ataaatgtgg cacgcatgga aagagtttga aatatataga tttcactagt
      661 gattatgcta gaaataatcc taatgggttt caggtacatg gaaaatcatt cttccattct
      721 aaacatgagc aaactgttat tggaataaaa tactgtgaaa gtattgaatc tggaaaaacc
      781 gtcaataaga aatcgcaact tatgtgccaa caaatgtata tgggcgaaaa accctttgga
      841 tgcagctgtt gtgagaaagc cttcagcagc aagtcatacc ttctagtgca tcagcaaact
      901 catgccgaag agaaacccta tggttgtaat gaatgtggga aagacttcag tagtaaatca
      961 tacctcattg tacatcagag aattcataca ggagagaaac tacatgaatg cagtgaatgc
     1021 aggaaaacat tcagtttcca ttcacagctt gttatacatc agagaattca cacaggtgag
     1081 aatccctatg agtgctgtga atgtgggaaa gtcttcagta ggaaagacca gcttgtttca
     1141 caccagaaaa ctcattcagg acagaaacca tatgtgtgta atgaatgtgg gaaagctttt
     1201 ggtttaaaat cacagctcat tatacatgaa agaattcata caggagagaa accatatgaa
     1261 tgcaatgaat gtcagaaagc ctttaataca aagtcaaacc ttatggtaca tcagagaacc
     1321 catacagggg agaaacctta tgtttgtagt gattgtggaa aagcctttac attcaagtca
     1381 cagctcattg tacatcaggg gattcacaca ggagtaaagc cctatgggtg tattcagtgt
     1441 ggtaaaggat tcagtttgaa atcacagctc attgtacatc agagaagtca cacaggaatg
     1501 aaaccttatg tatgcaatga atgtggcaaa gccttcagga gcaagtcata ccttattata
     1561 catacaagga ctcatacagg agaaaaactc catgaatgca acaattgtgg gaaagccttc
     1621 agttttaaat cacagctcat tatacatcag aggattcata caggagagaa cccctatgaa
     1681 tgccatgaat gtgggaaagc cttcagtcgg aaataccagc ttatttcaca ccagagaact
     1741 catgcaggag agaagcctta tgaatgcacc gactgtggaa aggcttttgg tttaaagtca
     1801 cagcttatta tacaccagag aactcataca ggggagaaac catttgaatg tagtgagtgt
     1861 cagaaagcct ttaatacaaa gtcaaacctg attgtacatc agagaactca tacaggagag
     1921 aaaccctata gttgtaatga atgtggaaaa gcctttacgt tcaaatcaca gctcattgta
     1981 cataaaggag tgcacactgg agtaaaaccc tatggatgca gtcaatgtgc aaaaaccttt
     2041 agtttgaagt cccagctcat tgtacatcag agaagtcaca caggagtaaa accatatgga
     2101 tgcagtgagt gtgggaaagc cttcaggagc aagtcatacc ttattataca tatgagaact
     2161 catacaggag agaaaccaca tgagtgcagg gaatgcggga aatcctttag tttcaattca
     2221 caactcattg tgcatcagag aattcacaca ggagaaaatc cctatgaatg cagtgaatgt
     2281 gggaaagcct ttaataggaa agaccagctc atttcacatc agcgaactca tgcaggggaa
     2341 aagccttatg ggtgcagtga atgtgggaaa gcttttagca gcaagtcata cctaattata
     2401 cacatgagaa ctcattcagg tgaaaaacca tatgaatgta atgaatgtgg gaaagccttc
     2461 atttggaaat cactactcat tgtacatgag cgaactcatg caggggtcaa cccttataaa
     2521 tgcagtcaat gtgagaaatc cttcagtggg aaattacgcc ttcttgtaca ccagagaatg
     2581 cacacaagag agaaaccata tgaatgcagt gagtgtggaa aagccttcat taggaattct
     2641 caactcattg tacatcaaag aactcattca ggagagaaac cctatgggtg caatgaatgt
     2701 gggaaaacct tctctcaaaa atcaattctc agtgcacatc agagaacaca tacaggagag
     2761 aagccttgta agtgcactga atgtgggaaa gccttttgtt ggaagtcaca gctcattatg
     2821 catcagagaa ctcatgtaga tgacaaacat tagtaatgaa ctagtccgta acttgaaagt
     2881 atttcgattt cttggcttta tatatcttgt ggaaaggacg agctagtccc acgtgcaggt
     2941 gtcag
//
