LOCUS       sequence-278374-        1682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2441
FEATURES             Location/Qualifiers
     source          1..1682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             390..401
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggcctcaaa cgattatacc caacaagcaa cccaaagcta tggggcctac
       61 cccacccagc ccgggcaggg ctattcccag cagagcagtc agccctacgg acagcagagt
      121 tacagtggtt atagccagtc cacggacact tcaggctatg gccagagcag ctattcttct
      181 tatggccaga gccagaacac aggctatgga actcagtcaa ctccccaggg atatggctcg
      241 actggcggct atggcagtag ccagagctcc caatcgtctt acgggcagca gtcctcctac
      301 cctggctatg gccagcagcc agctcccagc agcacctcgg gaagttacgg tagcagttct
      361 cagagcagca gctatgggca gccccagagt gggagctaca gccagcagcc tagctatggt
      421 ggacagcagc aaagctatgg acagcagcaa agctataatc cccctcaggg ctatggacag
      481 cagaaccagt acaacagcag cagtggtggt ggaggtggag gtggaggtgg aggtaactat
      541 ggccaagatc aatcctccat gagtagtggt ggtggcagtg gtggcggtta tggcaatcaa
      601 gaccagagtg gtggaggtgg cagcggtggc tatggacagc aggaccgtgg aggccgcggc
      661 aggggtggca gtggtggcgg cggcggcggc ggcggtggtg gttacaaccg cagcagtggt
      721 ggctatgaac ccagaggtcg tggaggtggc cgtggaggca gaggtggcat gggcggaagt
      781 gaccgtggtg gcttcaataa atttggtggc cctcgggacc aaggatcacg tcatgactcc
      841 gaacaggata attcagacaa caacaccatc tttgtgcaag gcctgggtga gaatgttaca
      901 attgagtctg tggctgatta cttcaagcag attggtatta ttaagacaaa caagaaaacg
      961 ggacagccca tgattaattt gtacacagac agggaaactg gcaagctgaa gggagaggca
     1021 acggtctctt ttgatgaccc accttcagct aaagcagcta ttgactggtt tgatggtaaa
     1081 gaattctccg gaaatcctat caaggtctca tttgctactc gccgggcaga ctttaatcgg
     1141 ggtggtggca atggtcgtgg aggccgaggg cgaggaggac ccatgggccg tggaggctat
     1201 ggaggtggtg gcagtggtgg tggtggccga ggaggatttc ccagtggagg tggtggcggt
     1261 ggaggacagc agcgagctgg tgactggaag tgtcctaatc ccacctgtga gaatatgaac
     1321 ttctcttgga ggaatgaatg caaccagtgt aaggccccta aaccagatgg cccaggaggg
     1381 ggaccaggtg gctctcacat ggggggtaac tacggggatg atcgtcgtgg tggcagagga
     1441 ggctatgatc gaggcggcta ccggggccgc ggcggggacc gtggaggctt ccgagggggc
     1501 cggggtggtg gggacagagg tggctttggc cctggcaaga tggattccag gggtgagcac
     1561 agacaggatc gcagggagag gccgtattag taatgaacta gtccgtaact tgaaagtatt
     1621 tcgatttctt ggctttatat atcttgtgga aaggacgact ccctcgagct agcttggatc
     1681 ag
//
