LOCUS       sequence-278488-        1859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2500
FEATURES             Location/Qualifiers
     source          1..1859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcctgc acccgcccga tctccgaggc cccagcagga ccccgcccgg
       61 ccccaggagc ccaccatgcc tccccccgag accccctctg aaggccgcca gcccagcccc
      121 agccccagcc ctacagagcg agcccccgct tcggaggagg agttccagtt tctgcgctgc
      181 cagcaatgcc aggcggaagc caagtgcccg aagctgctgc cttgtctgca cacgctgtgc
      241 tcaggatgcc tggaggcgtc gggcatgcag tgccccatct gccaggcgcc ctggccccta
      301 ggtgcagaca cacccgccct ggataacgtc tttttcgaga gtctgcagcg gcgcctgtcg
      361 gtgtaccggc agattgtgga tgcgcaggct gtgtgcaccc gctgcaaaga gtcggccgac
      421 ttctggtgct ttgagtgcga gcagctcctc tgcgccaagt gcttcgaggc acaccagtgg
      481 ttcctcaagc acgaggcccg gcccctagca gagctgcgca accagtcggt gcgtgagttc
      541 ctggacggca cccgcaagac caacaacatc ttctgctcca accccaacca ccgcacccct
      601 acgctgacca gcatctactg ccgaggatgt tccaagccgc tgtgctgctc gtgcgcgctc
      661 cttgacagca gccacagtga gctcaagtgc gacatcagcg cagagatcca gcagcgacag
      721 gaggagctgg acgccatgac gcaggcgctg caggagcagg atagtgcctt tggcgcggtt
      781 cacgcgcaga tgcacgcggc cgtcggccag ctgggccgcg cgcgtgccga gaccgaggag
      841 ctgatccgcg agcgcgtgcg ccaggtggta gctcacgtgc gggctcagga gcgcgagctg
      901 ctggaggctg tggacgcgcg gtaccagcgc gactacgagg agatggccag tcggctgggc
      961 cgcctggatg ctgtgctgca gcgcatccgc acgggcagcg cgctggtgca gaggatgaag
     1021 tgctacgcct cggaccagga ggtgctggac atgcacggtt tcctgcgcca ggcgctctgc
     1081 cgcctgcgcc aggaggagcc ccagagcctg caagctgccg tgcgcaccga tggcttcgac
     1141 gagttcaagg tgcgcctgca ggacctcagc tcttgcatca cccaggggaa agatgcagct
     1201 gtatccaaga aagccagccc agaggctgcc agcactccca gggaccctat tgacgttgac
     1261 ctggatgtct ccaatacaac gacagcccag aagaggaagt gcagccagac ccagtgcccc
     1321 aggaaggtca tcaagatgga gtctgaggag gggaaggagg caaggttggc tcggagctcc
     1381 ccggagcagc ccaggcccag cacctccaag gcagtctcac caccccacct ggatggaccg
     1441 cctagcccca ggagccccgt cataggaagt gaggtcttcc tgcccaacag caaccacgtg
     1501 gccagtggcg ccggggaggc agaggaacgc gttgtggtga tcagcagctc ggaagactca
     1561 gatgccgaaa actcgtcctc ccgagagctg gatgacagca gcagtgagtc cagtgacctc
     1621 cagctggaag gccccagcac cctcagggtc ctggacgaga accttgctga cccccaagca
     1681 gaagacagac ctctggtttt ctttgacctc aagattgaca atgaaagtgg gttctcctgg
     1741 ggctaccccc acccctttct aatttagtaa tgaactagtc cgtaacttga aagtatttcg
     1801 atttcttggc tttatatatc ttgtggaaag gacgaggaag cattctgtaa caagtgctt
//
