LOCUS       sequence-27849-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Lonergan KM, Iliopoulos O, Ohh M, Kamura T, Conaway RC, Conaway JW, 
            Kaelin WG
  TITLE     Regulation of hypoxia-inducible mRNAs by the von Hippel-Lindau tumor
            suppressor protein requires binding to complexes containing elongins
            B/C and Cul2.
  JOURNAL   Mol Cell Biol. 1998 Feb . 18(2):732-41.
  PUBMED    9447969
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Feb . 18(2):732-41."
COMMENT     Sequence Label: HA-VHL 1-167-pRc/CMV
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..31
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(565..583)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(566..583)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(603..620)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    609..720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cccctacgac gtgcccgact acgcctccct cggatccgcc accatgcccc ggagggcgga
       61 gaactgggac gaggccgagg taggcgcgga ggaggcaggc gtcgaagagt acggccctga
      121 agaagacggc ggggaggagt cgggcgccga ggagtccggc ccggaagagt ccggcccgga
      181 ggaactgggc gccgaggagg agatggaggc cgggcggccg cggcccgtgc tgcgctcggt
      241 gaactcgcgc gagccctccc aggtcatctt ctgcaatcgc agtccgcgcg tcgtgctgcc
      301 cgtatggctc aacttcgacg gcgagccgca gccctaccca acgctgccgc ctggcacggg
      361 ccgccgcatc cacagctacc gaggtcacct ttggctcttc agagatgcag ggacacacga
      421 tgggcttctg gttaaccaaa ctgaattatt tgtgccatct ctcaatgttg acggacagcc
      481 tatttttgcc aatatcacac tgccagtgta tactctgaaa gagcgatgcc tccaggttgt
      541 ccggtgaatt ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
      601 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
      661 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
      721 gcattgtctg antagnngtc attctantct gggggtgggg tggggcnnnc agcanggggg
      781 angatnggga acaatagcng gcatgctggg gatgcggngg gctnnnntgg nntnnnnnng
      841 gcggaangna
//
