LOCUS       sequence-278530-        4286 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2718
FEATURES             Location/Qualifiers
     source          1..4286
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccct gctgagatcc gcgcgttggt tgctgcgtgc cggggcggcc
       61 ccgcgcctcc cgctctccct gcgcctcctc cctggcggcc cgggccggct gcatgccgcc
      121 tcctatctgc ccgccgctcg cgccgggccc gtggccggag gactactgag cccagccagg
      181 ctgtatgcca ttgctgccaa agaaaaagat attcaagagg agtccacttt ttcttctagg
      241 aagatttcca atcagtttga ttgggctcta atgagactag atctttctgt tcgaagaact
      301 ggccgcattc caaagaagct tctacaaaaa gtttttaatg atacctgccg ctcaggtggc
      361 ctaggtggta gtcatgcctt gcttctacta cgtagttgtg gttctctctt gcctgaacta
      421 aagcttgaag agagaacaga atttgctcat aggatatggg acacacttca gaaattaggt
      481 gctgtgtatg atgtgagtca ctataatgct ttacttaaag tctatcttca aaatgaatat
      541 aaattctcac caactgattt cctggcaaaa atggaggaag caaacattca accaaatcga
      601 gtgacatacc agagattgat tgcttcttat tgtaatgtag gagatattga aggtgccagc
      661 aagattcttg gatttatgaa aactaaggat ctcccagtta cagaggcagt attcagtgcc
      721 cttgtgacag ggcatgccag agctggtgat atggagaatg cagaaaacat tctcacagtg
      781 atgagagatg ccggaattga gcctggtcca gacacatacc tcgcattatt gaatgcatat
      841 gctgagaagg gcgacattga ccatgttaag cagactctgg agaaggtgga gaagtccgag
      901 cttcacctta tggaccgtga tttactgcaa attattttta gcttcagtaa agctgggtat
      961 cctcagtatg tctcagaaat tttggaaaaa gttacatgtg aaagaagata tattccagat
     1021 gcaatgaacc tcattttact tttagtcact gaaaaattgg aagatgtagc gttgcaaatt
     1081 ttactagcat gccccgtatc aaaggaagat ggcccaagtg tctttggcag tttcttttta
     1141 caacactgtg tgactatgaa tacgcctgtg gagaagctaa cagactactg taagaagtta
     1201 aaggaagtcc agatgcactc ctttcctctg cagttcaccc tccattgtgc tttactcgcc
     1261 aataaaactg atttggcaaa agccttaatg aaggctgtga aggaggaagg ttttcctatc
     1321 agacctcact atttctggcc attgctagtt ggacgtcgga aggaaaaaaa tgttcaaggt
     1381 ataattgaaa tcctcaaagg aatgcaagaa ttgggagtac atcctgatca ggaaacatat
     1441 acagattatg tgattccatg ctttgatagt gtaaactcag cacgagccat tttgcaggaa
     1501 aatggatgtc tgtctgatag tgatatgttt tctcaagctg gattgagaag tgaagcagca
     1561 aatgggaact tagactttgt attatcattt ttgaaatcaa atacattgcc catctcgctg
     1621 cagtctataa gaagtagcct actgctaggc ttcaggaggt ctatgaatat aaatctttgg
     1681 agcgagataa cagaattgtt gtacaaggat ggacgttatt gccaggagcc tcgaggaccg
     1741 acggaagctg ttggctattt tctttataac ttgattgaca gcatgagtga ctcagaggta
     1801 caggccaagg aggagcattt gagacaatac ttccatcagc tggagaagat gaatgtaaaa
     1861 attcctgaaa atatctacag aggcattcgt aatctcctgg aaagctacca tgttcctgaa
     1921 ttgattaagg atgctcactt gttggttgag agtaagaatt tagactttca aaaaactgtg
     1981 caacttacat catctgaatt ggagtccaca cttgaaacac taaaagctga aaatcaacct
     2041 ataagagatg tcctaaagca actcatatta gtgctttgtt cagaagagaa tatgcaaaaa
     2101 gcccttgaat tgaaagcaaa atatgaatcc gacatggtta ctggtggcta tgcagcttta
     2161 ataaatttat gctgtcgaca tgataaagta gaagatgcct tgaacttgaa agaagaattt
     2221 gaccgcttag attcatctgc tgtccttgac accggcaagt atgtaggcct tgtaagagta
     2281 ttggcaaagc atggcaagct ccaagatgct attaacattc tgaaggagat gaaagagaag
     2341 gatgttctta tcaaagatac aacagccttg tcctttttcc acatgctaaa tggcgcagct
     2401 ttaagaggtg aaattgaaac agtaaaacag ttgcatgaag ccatcgtgac tctagggtta
     2461 gcagaaccat ccaccaacat aagtttccca ttggtcactg tacacttgga aaagggcgac
     2521 ctatctactg ctcttgaggt cgccattgac tgctatgaaa agtataaagt attaccaagg
     2581 attcatgatg tcttgtgtaa actggtagag aaaggcgaga ctgatctaat tcagaaagca
     2641 atggactttg tgagccaaga acaaggtgaa atggtgatgc tctatgatct cttctttgcc
     2701 ttcctacaaa caggaaatta caaagaggcc aagaagatca ttgagactcc agggattaga
     2761 gctcgatctg caaggcttca gtggttttgt gacagatgtg ttgcaaataa tcaggttgaa
     2821 actctggaaa aattagtgga gctgacacag aagctatttg aatgtgatag agaccagatg
     2881 tactacaatc tgctaaaact gtataaaata aacggtgact ggcaaagagc tgatgcagtc
     2941 tggaataaaa tccaagaaga aaatgttatt cctcgtgaaa agacattaag attattagca
     3001 gaaatcctta gagagggtaa ccaggaagtt ccgtttgacg tacctgagtt gtggtatgaa
     3061 gatgaaaaac attccctgaa ttcttcgtca gcctcaacca cagaacctga tttccagaaa
     3121 gatatattga ttgcctgccg attgaaccaa aaaaaagggg catatgatat tttcctgaat
     3181 gcaaaagagc aaaacattgt gtttaatgct gaaacctaca gcaatctcat taaattactg
     3241 atgtcagaag attattttac acaagcaatg gaagtgaaag cattcgcgga gacccacatc
     3301 aagggcttca cactgaacga tgctgccaac agccgcctca tcataacgca agttaggcgg
     3361 gattatttga aagaggctgt gacaacactg aaaacagtat tggatcagca gcagacccct
     3421 tctaggttag cagtgacccg tgtcatccag gcattggcca tgaagggtga tgttgaaaac
     3481 atagaagtag ttcagaagat gttaaatgga ctcgaagact ccattggact ttcaaaaatg
     3541 gttttcatca ataacattgc tttggctcaa ataaagaata ataacataga tgccgcaata
     3601 gaaaacattg aaaatatgct tacttcagag aataaagtca ttgaacccca atacttcggc
     3661 ttggcatact tattcagaaa agtaatagag gagcagttgg aaccagcagt tgaaaagata
     3721 agcatcatgg cggagagatt ggccaatcag tttgcaattt ataaacctgt cactgatttt
     3781 ttccttcaac ttgtggatgc aggcaaggtg gatgatgcca gagctctcct acagagatgt
     3841 ggtgcaattg ctgaacaaac cccgattttg ttgttgttcc tccttaggaa ttctaggaaa
     3901 caaggaaagg catcaactgt gaaatctgtg ttagaattga ttcctgaatt aaatgaaaag
     3961 gaagaagcat acaattccct catgaaaagc tatgtctcag agaaagatgt cacatctgct
     4021 aaagcactgt atgaacattt gactgcaaag aatacaaaat tggatgatct gtttctaaag
     4081 cgttacgcat ctttgctgaa gtatgctgga gagcctgtcc ctttcattga accccctgaa
     4141 agctttgaat tttatgcaca gcagctaaga aaattgaggg aaaactcttc ttagtaatga
     4201 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     4261 gatgggaagg actatgcctt aaccgg
//
