LOCUS       sequence-278533-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2719
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgg tctcctcaca gctgggcccc ggggatccac attcttcagc
       61 agtgtgacgg tagcttttgc acaggaaagg tggaggtgcc tcgtgtctac tccacgggac
      121 aggttcaagg aggggatacc aggaaagtcc aggagccttg tcctactagg acttccagtt
      181 tcccagcctg gcatgaactc ccagttggaa caaagggaag gcgcatggat gctggagggc
      241 gaagacctgc gaagtccctc tccaggctgg aagattatat ctggatcacc accagagcaa
      301 gccctttctg aagcttcatt ccaagaccca tgtgtagaga tgccccctgg ggattcagac
      361 cacgggacca gtgaccttga gaagagcttc aatctgagac cagtcctctc tccgcaacag
      421 agagtgcccg tggaagcgag acctcgcaaa tgtgagacac acaccgagag cttcaagaac
      481 tcggaaatcc tgaaacctca cagagcaaaa ccatatgcat gtaatgaatg tggcaaagcc
      541 ttcagttact gttcttccct ttctcagcat cagaagagcc acactggaga gaagccctat
      601 gagtgcagtg aatgtgggaa ggccttcagc cagagctcat ctctcattca gcaccagagg
      661 attcacactg gagagaagcc ttacaagtgc agtgaatgtg gaagagcctt cagccagaac
      721 gccaacctca ccaaacacca gcgaacccac accggagaga agccctacag atgcagcgag
      781 tgtgagaaag ccttcagtga ctgctcagct cttgttcagc atcagagaat tcataccgga
      841 gagaagccct acgaatgcag cgactgtggg aaggccttcc gtcacagtgc aaacctcacg
      901 aaccatcaga ggactcacac cggggagaag ccctacaagt gcagcgagtg tgggaaggcc
      961 ttcagttact gcgcagcgtt cattcagcac cagaggattc acaccgggga gaagccctac
     1021 agatgtgccg cgtgtgggaa ggccttcagc cagagtgcaa acctcacaaa ccatcagagg
     1081 actcacactg gggagaaacc ctacaagtgc agcgagtgtg ggaaggcctt cagccagagt
     1141 accaatctca taatccacca aaagacccac accggggaga agccatataa atgtaatgaa
     1201 tgtgggaaat tcttcagtga gagctcagcc ctcattcggc atcatataat ccacaccgga
     1261 gaaaaacctt atgagtgtaa tgagtgtggt aaagcgttta accagagctc atcccttagt
     1321 cagcatcaga gaatccacac aggcgtgaaa ccctacgaat gcagcgagtg tgggaaggcc
     1381 ttccggtgca gctctgcctt cgttagacat cagagactcc acgccggaga gtagtaatga
     1441 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1501 gactagttgt tggcctaggc gttaac
//
