LOCUS       sequence-278545-        2891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2736
FEATURES             Location/Qualifiers
     source          1..2891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1617..1626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgatagataa gaaagccatg tacaaagcag gaatggtaca gagagtccaa
       61 aatgaggtga aaatacattg ccaattgaaa catccttcta tcttggagct ttataactat
      121 tttgaagata gcaattatgt gtatctggta ttagaaatgt gccataatgg agaaatgaac
      181 aggtatctaa agaatagagt gaaacccttc tcagaaaatg aagctcgaca cttcatgcac
      241 cagatcatca cagggatgtt gtatcttcat tctcatggta tactacaccg ggacctcaca
      301 ctttctaacc tcctactgac tcgtaatatg aacatcaaga ttgctgattt tgggctggca
      361 actcaactga aaatgccaca tgaaaagcac tatacattat gtggaactcc taactacatt
      421 tcaccagaaa ttgccactcg aagtgcacat ggccttgaat ctgatgtttg gtccctgggc
      481 tgtatgtttt atacattact tatcgggaga ccacccttcg acactgacac agtcaagaac
      541 acattaaata aagtagtatt ggcagattat gaaatgccat cttttttgtc aatagaggcc
      601 aaggacctta ttcaccagtt acttcgtaga aatccagcag atcgtttaag tctgtcttca
      661 gtattggacc atccttttat gtcccgaaat tcttcaacaa aaagtaaaga tttaggaact
      721 gtggaagact caattgatag tgggcatgcc acaatttcta ctgcaattac agcttcttcc
      781 agtaccagta taagtggtag tttatttgac aaaagaagac ttttgattgg tcagccactc
      841 ccaaataaaa tgactgtatt tccaaagaat aaaagttcaa ctgatttttc ttcttcagga
      901 gatggaaaca gtttttatac tcagtgggga aatcaagaaa ccagtaatag tggaagggga
      961 agagtaattc aagatgcaga agaaaggcca cattctcgat accttcgtag agcttattcc
     1021 tctgatagat ctggcacttc taatagtcag tctcaagcaa aaacatatac aatggaacga
     1081 tgtcactcag cagaaatgct ttcagtgtcc aaaagatcag gaggaggtga aaatgaagag
     1141 aggtactcac ccacagacaa caatgccaac atttttaact tctttaaaga aaagacatcc
     1201 agtagttctg gatcttttga aagacctgat aacaatcaag cactctccaa tcatctttgt
     1261 ccaggaaaaa ctccttttcc atttgcagac ccgacacctc agactgaaac cgtacaacag
     1321 tggtttggga atctgcaaat aaatgctcat ttaagaaaaa ctactgaata tgacagcatc
     1381 agcccaaacc gggacttcca gggccatcca gatttgcaga aggacacatc aaaaaatgcc
     1441 tggactgata caaaagtcaa aaagaactct gatgcttctg ataatgcaca ttctgtaaaa
     1501 cagcaaaata ccatgaaata tatgactgca cttcacagta aacctgagat aatccaacaa
     1561 gaatgtgttt ttggctcaga tcctctttct gaacagagca agactagggg tatggagcca
     1621 ccatggggtt atcagaatcg tacattaaga agcattacat ctccgttggt tgctcacagg
     1681 ttaaaaccaa tcagacagaa aaccaaaaag gctgtggtga gcatacttga ttcagaggag
     1741 gtgtgtgtgg agcttgtaaa ggagtatgca tctcaagaat atgtgaaaga agttcttcag
     1801 atatctagtg atggaaatac gatcactatt tattatccaa atggtggtag aggttttcct
     1861 cttgctgata gaccaccctc acctactgac aacatcagta ggtacagctt tgacaattta
     1921 ccagaaaaat actggcgaaa atatcaatat gcttccaggt ttgtacagct tgtaagatct
     1981 aaatctccca aaatcactta ttttacaaga tatgctaaat gcattttgat ggagaattct
     2041 cctggtgctg attttgaggt ttggttttat gatggggtaa aaatacacaa aacagaagat
     2101 ttcattcagg tgattgaaaa gacagggaag tcttacactt taaaaagtga aagtgaagtt
     2161 aatagcttga aagaggagat aaaaatgtat atggaccatg ctaatgaggg tcatcgtatt
     2221 tgtttagcac tggaatccat aatttcagaa gaggaaagga aaactaggag tgctcccttt
     2281 ttcccaataa tcataggaag aaaacctggt agtactagtt cacctaaggc cttatcacct
     2341 cctccttctg tggattcaaa ttacccaacg agagagagag catctttcaa cagaatggtc
     2401 atgcatagtg ctgcttctcc aacacaggca ccaatcctta atccctctat ggttacaaat
     2461 gaaggacttg gtcttacaac tacagcttct ggaacagaca tctcttctaa tagtctaaaa
     2521 gattgtcttc ctaaatcagc acaacttttg aaatctgttt ttgtgaaaaa tgttggttgg
     2581 gctacacagt taactagtgg agctgtgtgg gttcagttta atgatgggtc ccagttggtt
     2641 gtgcaggcag gagtgtcttc tatcagttat acctcaccaa atggtcaaac aactaggtat
     2701 ggagaaaatg aaaaattacc agactacatc aaacagaaat tacagtgtct gtcttccatc
     2761 cttttgatgt tttctaatcc gactcctaat tttcattagt aatgaactag tccgtaactt
     2821 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac ttagaagctg
     2881 gattccttcg t
//
