LOCUS       sequence-27855-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  TITLE     Gail Martin plasmids
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mFGF2
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(544..560)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(545..561)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(589..607)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(628..644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(628..644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(641..663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    652..668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(676..682,683..700,701..706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    721..742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc gccgcggaag ggccatggct gccagcggca
       61 tcacctcgct tcccgcactg ccggaggacg gcggcgccgc cttcccacca ggccacttca
      121 aggaccccaa gcggctctac tgcaagaacg gcggcttctt cctgcgcatc catcccgacg
      181 gccgcgtgga tggcgtccgc gagaagagcg acccacacgt caaactacaa ctccaagcag
      241 aagagagagg agttgtgtct atcaagggag tgtgtgccaa ccggtacctt gctatgaagg
      301 aagatggacg gctgctggct tctaagtgtg ttacagaaga gtgtttcttc tttgaacgac
      361 tggaatctaa taactacaat acttaccggt cacggaaata ctccagttgg tatgtggcac
      421 tgaaacgaac tgggcagtat aaactcggat ccaaaacggg acctggacag aaggccatac
      481 tgtttcttcc aatgtctgct aagagctgac tcacgggctg caggaattcg atatcaagct
      541 tatcgatacc gtcgacctcg agggggggcc cggtaccagc ttttgttccc tttagtgagg
      601 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      781 cctgtcgtgc
//
