LOCUS       sequence-278585-         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0148
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagccccc caagatgaac ccagtggtgg agccactgtc ctggatgctg
       61 ggcacctggc tgtcggaccc acctggagcc gggacctacc ccacactgca gcccttccag
      121 tacctggagg aggttcacat ctcccacgtg ggccagccca tgctgaactt ctcgttcaac
      181 tccttccacc cggacacgcg caagccgatg cacagagagt gtggcttcat tcgcctcaag
      241 cccgacacca acaaggtggc ctttgtcagc gcccagaaca caggcgtggt ggaagtggag
      301 gagggcgagg tgaacgggca ggagctgtgc atcgcatccc actccatcgc caggatctcc
      361 ttcgccaagg agccccacgt agagcagatc acccggaagt tcaggctgaa ttctgaaggc
      421 aaacttgagc agacggtctc catggcaacc acgacacagc caatgactca gcatcttcac
      481 gtcacctaca agaaggtgac cccgtagtaa tgaactagtc cgtaacttga aagtatttcg
      541 atttcttggc tttatatatc ttgtggaaag gacgagtccc gaatcgcgat gactaggag
//
