LOCUS       sequence-278615-        4814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2768
FEATURES             Location/Qualifiers
     source          1..4814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaccggg gtgtgacgag ttcctgccgc caccggagtg cccggttttt
       61 gagcctagct gggctgaatt ccaagacccg cttggctaca ttgcgaaaat aaggcccata
      121 gcagagaagt ctggcatctg caaaatccgc ccacccgcgg attggcagcc tccttttgca
      181 gtagaagttg acaatttcag atttactcct cgcgtccaaa ggctaaatga actggaggcc
      241 caaactagag tgaaattgaa ctatttggat cagattgcaa aattctggga aattcaaggc
      301 tcctctttaa agattcccaa tgtggagcgg aagatcttgg acctctacag ccttagtaag
      361 attgtgattg aggaaggtgg ctatgaagcc atctgcaagg atcgtcggtg ggctcgagtt
      421 gcccagcgtc tccactaccc accaggcaaa aacattggct ccctgctacg atcacattac
      481 gaacgcatta tttaccccta tgaaatgttt cagtctggag ccaaccatgt gcaatgtaac
      541 acacacccgt ttgacaatga ggtaaaagat aaggaataca agccccacag catccccctt
      601 agacagtctg tgcagccttc aaagttcagc agctacagtc gacgggcaaa aaggctacag
      661 cctgatccag agcctacaga ggaggacatt gagaagcatc cagagctaaa gaagttacag
      721 atatatgggc caggtcccaa aatgatgggc ttgggcctta tggctaagga taaggataag
      781 actgtgcata agaaagtcac atgcccccca actgttacgg tgaaggatga gcaaagtgga
      841 ggtgggaacg tgtcatcaac attgctcaag cagcacttga gcctagagcc ctgcactaag
      901 acaaccatgc aacttcgaaa gaatcacagc agtgcccagt ttattgactc atatatttgc
      961 caagtatgct cccgtgggga tgaagatgat aagcttcttt tctgtgatgg ctgtgatgac
     1021 aattaccaca tcttctgctt gttaccaccc cttcctgaaa tccccagagg catctggagg
     1081 tgcccaaaat gtatcttggc ggagtgtaaa cagcctcctg aagcttttgg atttgaacag
     1141 gctacccagg agtacagttt gcagagtttt ggtgaaatgg ctgattcctt caagtccgac
     1201 tacttcaaca tgcctgtaca tatggtgcct acagaacttg tagagaagga attctggagg
     1261 ctggtgagca gcattgagga agacgtgaca gttgaatatg gagctgatat tcattccaaa
     1321 gaatttggca gtggctttcc tgtcagcaat agcaaacaaa acttatctcc tgaggagaag
     1381 agacaaagtc tcactgtgtt gaccaggctg atctcaagct tctgggctca agcagtcctc
     1441 ccaccttggc ctcccaaagt gttgggatta caggagtatg cgaccagtgg ttggaacctg
     1501 aatgtgatgc cagtgctaga tcagtctgtt ctctgtcaca tcaatgcaga catctcaggc
     1561 atgaaggtgc cctggctgta cgtgggcatg gttttctcag cattttgttg gcatattgag
     1621 gatcactgga gttactctat taactatctg cattggggtg agccgaagac ctggtatggt
     1681 gtaccctccc tggcagcaga gcatttggag gaggtgatga agatgctgac acctgagctg
     1741 tttgatagcc agcctgatct cctacaccag cttgtcactc tcatgaatcc caacactttg
     1801 atgtcccatg gtgtgccagt tgtccgcaca aaccagtgtg caggggagtt tgtcatcact
     1861 tttcctcgtg cttaccacag tggttttaac caaggctaca attttgctga agctgtcaac
     1921 ttttgtactg ctgactggct acctgctgga cgccagtgca ttgaacacta ccgccggctc
     1981 cggcgctatt gtgtcttctc ccacgaggag ctcatctgca agatggctgc cttcccagag
     2041 acgttggatc tcaatctagc agtagctgtg cacaaggaga tgttcattat ggttcaggag
     2101 gagcgacgtc tacgaaaggc ccttttggag aagggcgtca cggaggctga gcgagaggct
     2161 tttgagctgc tcccagatga tgaacgccag tgcatcaagt gcaagaccac gtgcttcttg
     2221 tcagccctgg cctgctacga ctgcccagat ggccttgtat gcctttccca catcaatgac
     2281 ctctgcaagt gctctagtag ccgacagtac ctccggtatc ggtacacctt ggatgagctc
     2341 cccaccatgc tgcataaact gaagattcgg gctgagtctt ttgacacctg ggccaacaaa
     2401 gtgcgagtgg ccttggaggt ggaggatggc cgtaaacgca gctttgaaga gctaagggca
     2461 ctggagtctg aggctcgtga gaggaggttt cctaatagtg agctgcttca gcgactgaag
     2521 aactgcctga gtgaggtgga ggcttgtatt gctcaagtcc tggggctggt cagtggtcag
     2581 gtggccagga tggacactcc acagctgacc ttgactgaac tccgggtcct tcttgagcag
     2641 atgggcagcc tgccctgtgc catgcatcag attggggatg tcaaggatgt cctggaacag
     2701 gtggaggcct atcaagctga ggctcgtgag gctctggcca cactgccctc tagtccaggg
     2761 ctattgcggt ccctgttgga gagggggcag cagctgggtg tagaggtgcc tgaagcccat
     2821 cagcttcagc agcaggtgga gcaggcgcaa tggctagatg aagtgaagca ggccctggcc
     2881 ccttctgctc acaggggctc tctggtcatc atgcaggggc ttttggttat gggtgccaag
     2941 atagcctcca gcccttctgt ggacaaggcc cgggctgagc tgcaagaact actgaccatt
     3001 gcagagcgct gggaagaaaa ggctcatttc tgcctggagg ccaggcagaa gcatccacca
     3061 gccacattgg aagccataat tcgtgagaca gaaaacatcc ctgttcacct gcctaacatc
     3121 caggctctca aagaagctct gactaaggca caagcttgga ttgctgatgt ggatgagatc
     3181 caaaatggtg accactaccc ctgtctagat gacttggagg gcctggtggc tgtgggccgg
     3241 gacctgcctg tggggctgga agagctgaga cagctagagc tgcaggtatt gacagcacat
     3301 tcctggagag agaaggcctc caagaccttt ctcaagaaga attcttgcta cacactgctt
     3361 gaggtgcttt gcccgtgtgc agacgctggc tcagacagca ccaagcgtag ccggtggatg
     3421 gagaaggcgc tggggttgta ccagtgtgac acagagctgc tggggctgtc tgcacaggac
     3481 ctcagagacc caggctctgt gattgtggcc ttcaaggaag gggaacagaa ggagaaggag
     3541 ggtatcctgc agctgcgtcg caccaactca gccaagccca gtccactggc accatccctc
     3601 atggcctctt ctccgacttc tatctgtgtg tgtgggcagg tgccagctgg ggtgggagtt
     3661 ctgcagtgtg acctgtgtca ggactggttc catgggcagt gtgtgtcagt gccccatctc
     3721 ctcacctctc caaagcccag tctcacttca tctccactgc tagcctggtg ggaatgggac
     3781 acaaaattcc tgtgtccact gtgtatgcgc tcacgacggc cacgcctaga gacaatccta
     3841 gccttgctgg ttgccctgca gaggctgccc gtgcggctgc ctgagggtga ggcccttcag
     3901 tgtctcacag agagggccat tggctggcaa gaccgtgcca gaaaggctct ggcctctgaa
     3961 gatgtgactg ctctgttgcg acagctggct gagcttcgcc aacagctaca ggccaaaccc
     4021 agaccagagg aggcctcagt ctacacttca gccactgcct gtgaccctat cagagaaggc
     4081 agtggcaaca atatttctaa ggtccaaggg ctgctggaga atggagacag tgtgaccagt
     4141 cctgagaaca tggctccagg aaagggctct gacctggagc tactgtcctc actgttgccg
     4201 cagttgactg gccctgtgtt ggagctgcct gaggcaatcc gggctcccct ggaggagctc
     4261 atgatggaag gggacctgct tgaggtgacc ctggatgaga accacagcat ctggcagctg
     4321 ctgcaggctg gacagcctcc agacctggac agaattcgca cacttctgga gctggaaaaa
     4381 tttgaacatc aagggagtcg gacaaggagc cgggctctgg agaggcgacg gcggcggcag
     4441 aaggtggatc agggtagaaa cgttgagaat cttgttcaac aggagcttca gtcaaaaagg
     4501 gctcggagct cagggattat gtctcaggtg ggccgagaag aagaacatta tcaggagaaa
     4561 gcagaccgtg aaaatatgtt cctgacacct tccacagacc acagcccttt cttgaaagga
     4621 aaccaaaata gcttacaaca caaggattca ggctcttcag ctgcttgtcc ttctttaatg
     4681 cctttgctac aactctccta ctctgatgag caacagttgt agtaatgaac tagtccgtaa
     4741 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga atatggagtg
     4801 aagtttagct caat
//
