LOCUS       sequence-278618-        4241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2774
FEATURES             Location/Qualifiers
     source          1..4241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    808..819
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgccacca tggagccggg gtccgacgat ttcctaccgc caccggagtg cccggtgttc
       61 gagcctagct gggccgagtt ccgagaccct cttggctaca tcgcgaaaat caggcccatc
      121 gcagagaaat cgggcatttg caagatccgc ccacccgcga ttgtggtgga ggaaggtggt
      181 tatgaagcta tctgcaagga ccgtcggtgg gctcgggtag cccagcgcct caactatcca
      241 ccaggcaaaa atattggctc cttgctacgc tcccactacg aacgcattgt ttatccctat
      301 gaaatgtacc agtctggagc caaccttgtg cagtgtaaca cacgtccatt tgataatgag
      361 gagaaggaca aggaatacaa accccacagc atccccctac gacagtctgt gcagccttcc
      421 aagttcaaca gctatggccg gcgggccaag agactgcagc ctgatccgga acccacagag
      481 gaagacattg agaagaatcc agagctgaaa aagctacaga tctatggggc aggccccaag
      541 atgatgggcc tgggcctcat ggccaaagac aagactctgc ggaagaaaga taaggagggg
      601 cctgagtgtc cccccacagt agtggtgaag gaggagttag gtggggatgt gaaggtggag
      661 tcaacatcgc ctaagacctt cctggagagc aaggaggagc tgagtcacag cccagaaccc
      721 tgcaccaaga tgaccatgag gctacggagg aaccacagca atgcccagtt tattgagtca
      781 tatgtctgcc ggatgtgttc tcgaggggat gaggatgaca agctcctgct gtgtgatggc
      841 tgtgatgaca actaccacat cttctgcctg ctgcctcctc tgcctgagat ccccaagggt
      901 gtctggcggt gcccaaagtg tgtcatggcg gagtgtaagc ggcccccaga agcctttggc
      961 tttgagcagg ctacccggga atacactctg cagagctttg gcgagatggc cgactccttt
     1021 aaagctgact acttcaacat gcccgtgcat atggtgccca cagaacttgt ggagaaggag
     1081 ttctggaggc tggtaaatag cattgaggaa gatgtgactg ttgagtatgg agctgacatc
     1141 cattccaaag aatttggcag cggtttccct gtcagtgaca gtaaacggca cctaaccccc
     1201 gaagaggagg agtatgctac cagtggttgg aacctaaatg tgatgccggt gttggaacag
     1261 tctgtactgt gccacatcaa tgcagatatc tctggcatga aggtgccctg gctctacgtg
     1321 ggcatggtct tctcagcctt ttgctggcat attgaggatc actggagtta ctccattaac
     1381 tacctccact ggggtgagcc gaagacctgg tatggggtgc cctcacttgc agcagaacat
     1441 ttggaagaag tgatgaagaa gctgacacct gaactatttg atagccagcc tgacctcctg
     1501 caccaacttg tcaccctcat gaatcccaac accctcatgt cccatggtgt gccagttgtc
     1561 cgcacaaacc agtgtgcagg agagtttgtc atcaccttcc cccgtgctta ccacagcggc
     1621 ttcaaccaag gctacaactt tgccgaggct gtcaactttt gcactgctga ctggttgcct
     1681 gctgggcgcc agtgcattga gcactaccgc cggctccgga gatactgcgt cttctcccat
     1741 gaggagctta tctgcaagat ggctgcctgc ccagagaagc tagacctgaa cctggcggca
     1801 gctgtgcata aggagatgtt catcatggtg caagaagagc ggcgtctacg aaaggccctg
     1861 ctggagaagg gtatcacaga ggctgagcga gaggctttcg agctgctccc agatgatgag
     1921 cgccagtgta tcaagtgcaa gactacgtgt ttcctgtcag ccctggcctg ctacgactgc
     1981 ccagacggcc ttgtctgcct ttcccacatc aatgatctct gcaagtgctc cagtagccgg
     2041 cagtacctgc ggtatcggta taccttggat gagcttcctg ccatgctgca taagctgaag
     2101 gttcgggctg agtcctttga cacctgggcc aacaaagtgc gagtggccct ggaggtggag
     2161 gatgggcgga agcgcagcct tgaagaactg agggcactag agtctgaagc ccgtgagcgg
     2221 aggtttccta atagtgagct gctgcagcaa ctaaagaact gcctgagtga ggcagaggct
     2281 tgcgtgtccc gagctctggg actggtcagc ggccaggaag ctggccccca cagggtggct
     2341 ggtctacaga tgaccctgac tgagctccgg gcctttctgg accagatgaa caacctgcct
     2401 tgcgccatgc accagattgg ggatgtcaag ggtgttctgg aacaggtgga ggcctaccag
     2461 gctgaggctc gtgaggccct ggcctcactg ccctccagtc cagggctact gcagtccctg
     2521 ttggagaggg ggcggcagct gggggtggag gtgcctgagg cccagcagct ccagcggcag
     2581 gtggaacagg cgcgatggct ggatgaggtg aaacgcacac tggccccctc agcccgaagg
     2641 ggcaccttgg ctgtcatgcg aggactgttg gtcgcgggtg ccagtgtagc ccctagccct
     2701 gctgtggata aagcccaggc cgagctgcag gaactgctga ccattgctga acgctgggag
     2761 gagaaagccc acctctgcct ggaggccagg cagaagcatc caccagccac acttgaggcc
     2821 ataatccgtg aagcggaaaa catccctgtt cacctgccca acatccaggc tctcaaggag
     2881 gctcttgcta aggcccgggc ctggattgct gatgttgatg agatccaaaa tggtgaccac
     2941 tacccctgcc tggatgactt ggagggccta gtagctgtgg gccgggacct acctgtgggg
     3001 ctggaggagc tgagacagct agagctacag gtactgacag cgcactcctg gagggagaag
     3061 gcctccaaga ccttcctcaa gaaaaattct tgctacacgc tgctggaggt tctctgccca
     3121 tgtgcagatg ccggctcaga cagcaccaag cgcagccggt ggatggagaa ggagctgggg
     3181 ttgtacaaat ctgacacaga gctgctgggg ctgtctgcgc aggacctcag ggacccaggc
     3241 tctgtgatcg tggccttcaa ggagggggaa cagaaggaga aggagggtat cctgcagctg
     3301 cgtcgcacca attcggccaa gcccagtcca ctggcatcat cgagcacggc ctcctctaca
     3361 acctctatct gtgtgtgtgg gcaggtgctg gctggggcgg gagctctgca gtgtgacctg
     3421 tgtcaggact ggttccatgg gcggtgtgtg tcagtgcctc gcctcctcag ctctccgagg
     3481 cccaatccca cctcatcccc actgctggcc tggtgggaat gggacaccaa attcctgtgt
     3541 ccactgtgta tgcgctcaag gcgcccgcgc ctggagacca tcctggcact gctggtagcc
     3601 ctgcagagac tgcctgtgcg gctgcccgag ggcgaggccc tgcagtgcct cacagagagg
     3661 gccatcagct ggcaaggccg cgccaggcag gctctggcct ctgaagatgt gactgctctt
     3721 ttgggacggc tggctgagct ccgccaacgg ctacaggctg aacctagacc tgaggagcct
     3781 cctaactacc ctgcagcccc tgcttctgac cccctcagag agggcagtgg caaggatatg
     3841 cctaaggtcc agggcttact ggagaatgga gacagtgtga ccagtcctga gaaggtagcc
     3901 ccggaggagg gctcagatct ggagctgctg tcctcgctgt tgccacagtt gactggccct
     3961 gtgttggaac tgcctgaggc aacccgggcc cccttggagg agctcatgat ggagggggac
     4021 ctgctcgagg tgaccctgga tgagaaccac agcatccctg agtcccttga cttttgtatt
     4081 ctgactccaa ggtattgttc agacctcagc tcctgggggc cggcccctgg agtcttccct
     4141 ccctggtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     4201 tcttgtggaa aggacgaatt tgatcattaa gttccctcga c
//
