LOCUS       sequence-278621-        1973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2780
FEATURES             Location/Qualifiers
     source          1..1973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccataa atatgtgggt ttgcagtatc acggatcagt gacatttgag
       61 gatgtggcca tagccttctc ccagcaggag tgggagagtc tggactcttc ccagaggggc
      121 ttgtacagag atgtgatgtt ggagaactac aggaacttgg tgtcaatggg acattcccgt
      181 tctaaaccac atgtgatcgc cttattggaa caatggaaag agcctgaagt gacagtgagg
      241 aaagatggaa gaagatggtg cacagatttg cagttggaag atgatacaat cggctgtaaa
      301 gaaatgccca cctctgaaaa ctgtccatct tttgctctac atcagaaaat aagtagacag
      361 aaaccacgtg aatgtcagga atatggaaag accctttgtc aagactcaaa gcctgttcaa
      421 catgaaagaa tacatagtag tgaaaaaccc aacagatgta aagaatgtgg gaagaacttt
      481 agtaatggac atcaactcac catacatcag agattgcatg ttggtgagaa accctataaa
      541 tatgaaaaat gtgggaaggc ctttatcagt ggctcagcct ttgttaagca tgggagaatt
      601 cacactggtg agaagccact caaatgtaag caatgtggaa agactattag tggtagctat
      661 caacttacag tacataagag tattcatact gggaagaaac catatgagtg cggggaatgt
      721 gggaaagctt ttctagtata tggaaagctt acccggcatc agagtactca cactggtgaa
      781 aaaccctttg ggtgtgagga gtgtgggaag gccttcagta ccttttcata cctggttcaa
      841 catcagcgaa ttcataccag tgaaaaacct tacgaatgca aagaatgtgg gaaggccttt
      901 agcactagct caccccttgc taagcatcag agaattcata ctggcgagaa accctatgaa
      961 tgtaaggagt gtgggaagtc cttcactgtg tatggacagc ttactcgaca tcagagtatt
     1021 catactggtg agaaaccttt tgaatgtaag gaatgtggaa aggcctttag acttagttcc
     1081 ttccttcatg cacatcagcg aattcatgca gagataaagc cctacggatg caaggaatgc
     1141 gggagaacct tcagtcgtgc ctcatatctt gttcaacatg gaagacttca cactggcgag
     1201 aagccctatg aatgtaagga gtgtggcaag gcctttagta ctggctcata ccttgttcag
     1261 catcagagga tccatactgg ggagaaaccc tatgaatgta aggaatgtgg caaagccttt
     1321 attagtcgcc atcagcttac cgtacatcaa agggttcata ctggagagaa accctatgag
     1381 tgtaaggaat gtggcaaggc cttcagagtg cacgtacatc tcacacagca tcggaaaatt
     1441 catactgatg taaagcccta tgaatgtaag gaatgtggaa agacttttag tcgagcctcg
     1501 taccttgtac aacatagcag aatccatact ggtaagaagc cctatgagtg taaggagtgt
     1561 ggcaaggcct tcagttctgg ctcatacctt gttcagcatc aaagaattca tactggggag
     1621 aaaccctatg aatgtaacaa atgtgggaaa gcctttactg tttatggaca acttattgga
     1681 catcagagtg ttcacactgg tgagaaacct tttgaatgta aggaatgcgg gaaggccttt
     1741 agacttaatt cattccttac tgaacatcag cgggtacaca ctggtgagaa accctttaaa
     1801 tgcaaaaaat gtgggaagac ctttagatac agttcagccc ttaaagtgca tctgagaaaa
     1861 catatgagtg ttatacccta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1921 tggctttata tatcttgtgg aaaggacgag ctagtacccg agtctatgga tga
//
