LOCUS       sequence-278648-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2896
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagatgaa ggtctgggac ccagacaacc ctctcacaga ccggcagatc
       61 gaccagtttc ttgtggtggc ccgagctgtg ggaacctttg caagagccct agattgtagc
      121 agctccattc ggcagccaag cttgcacatg agtgcagctg ctgcctcccg agatatcact
      181 ctgtttcacg ccatggatac cttgcaaagg aacggctacg acctggctaa ggccatgtcg
      241 accctggtac cccagggagg cccggtgctg tgtcgggatg agatggagga atggtcagcc
      301 tcagaggcca tgctatttga ggaggcccta gagaagtatg ggaaggactt caatgatatt
      361 cgccaggatt ttctaccctg gaagtcactt gccagcatag tccagtttta ttacatgtgg
      421 aaaaccacag accggtatat tcagcagaaa aggttgaaag ctgctgaagc agacagcaaa
      481 ctgaaacagg tctacattcc cacctacact aagccaaacc ctaaccagat catttctgtg
      541 ggttcaaaac ctggcatgaa tggggctgga tttcagaagg gcctgacttg tgagagttgc
      601 cacaccacac agtctgctca gtggtatgcc tggggcccac ctaacatgca gtgccgcctc
      661 tgtgcttcct gttggatcta ctggaagaag tatgggggac tgaagacccc aactcagctt
      721 gagggggcca ctcggggcac cacggagcca cactcaaggg gtcatttatc cagacctgaa
      781 gctcaaagtc tctctcctta cacaaccagc gccaacaggg ccaagctact ggctaagaac
      841 agacaaactt tcctgcttca gaccacaaag ctgacccgtc ttgccagacg catgtgcagg
      901 gacctattac agccaaggag ggccgcccga cggccttatg ctcctatcaa tgccaatgcc
      961 atcaaagcag agtgctccat tcgacttcct aaggccgcca agactccatt gaagattcac
     1021 cctctggtgc ggctgcccct ggcaactatc gtcaaagatc tggtggccca ggcacccctg
     1081 aaaccaaaaa cacctcgggg taccaagaca ccgatcaaca gaaaccagct gtcccagaac
     1141 cggggactgg ggggcattat ggtgaaacgg gcctatgaga ctatggcagg ggcaggggtt
     1201 cctttctctg ccaatggaag gcctctggct tcagggattc gttcaagctc acagccagca
     1261 gccaagcgtc agaaactaaa cccagctgat gcccccaatc ctgtggtgtt tgtggccaca
     1321 aaggatacca gggccctacg gaaggctctg acccatctgg aaatgcggcg agctgctcgc
     1381 cgacccaact tgcccctgaa ggtgaagcca acgctgattg cagtgcggcc ccctgtccct
     1441 ctacctgcac cctcacatcc tgccagcacc aatgagccta ttgtcctgga ggactagtaa
     1501 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1561 gacgatctat acctgcttgg cgccaacac
//
