LOCUS       sequence-27866-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Meyers EN, Lewandoski M, Martin GR
  TITLE     An Fgf8 mutant allelic series generated by Cre- and Flp-mediated 
            recombination.
  JOURNAL   Nat Genet. 1998 Feb . 18(2):136-41.
  PUBMED    9462741
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            1998 Feb . 18(2):136-41."
COMMENT     Sequence Label: pK-11
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..48
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        64..563
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(99..122)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     496..515
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(640..659)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccgcggtggc ggccgaagtt cctattctct agaaagtata ggaacttcgc ggccaattct
       61 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
      121 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
      181 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
      241 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
      301 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
      361 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
      421 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
      481 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
      541 cttcctcatc tccgggcctt tcgacctgca gccaatatgg gatcggccat tgaacaagat
      601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
//
