LOCUS       sequence-278660-        4469 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2088
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcggtgc ttgcagctcc tttaccacct cttccagccc taccatttat
       61 tctacctcag tcaccgacag caaggctatg caagtggaga gctgctcctc agccgtgggg
      121 gtaagtaaca gaggggtaag tgaaaagcag ttaaccagta acacagttca gcagcatcca
      181 tcaacaccga agaggcacac agtcttgtac atctcaccac cacctgagga cttgctggat
      241 aacagtcgga tgtcctgcca ggatgagggg tgtggattgg aatctgagca gagctgcagt
      301 atgtggatgg aggattcccc ctccaacttc agtaacatga gcaccagttc ctacaatgat
      361 aacactgagg tacctcgtaa atcacgaaaa cgaaatccaa agcagaggcc gggggtcaaa
      421 cgacgagatt gtgaagaatc taatatggat atatttgatg ccgacagtgc caaagcacct
      481 cactatgtgc tttctcagct taccacggac aacaaaggca actcaaaagc gggaaatgga
      541 acattggaaa accaaaaagg aactggagta aagaagagcc ctatgttgtg tggacaatat
      601 cctgttaaaa gtgagggaaa ggagctgaag atagttgtac aacctgagac acagcaccga
      661 gctcggtacc tgactgaggg cagccgtggc tcagtgaaag atagaacaca gcaaggcttt
      721 cctacagtaa agctggaagg ccataatgaa cctgtagtgt tgcaagtgtt tgtgggcaac
      781 gactctggac gagtgaaacc acatggattt tatcaggcct gcagagtaac tggacgaaat
      841 acaactcctt gcaaagaagt ggacattgaa ggcactactg ttatagaagt cggccttgat
      901 cctagcaaca acatgacact ggcggtggac tgcgtaggga tattgaaatt gaggaatgct
      961 gatgtcgaag ccagaatagg aattgctggt tccaagaaga aaagcactcg tgccagattg
     1021 gtttttcgag ttaatatcat gaggaaagat ggctccactt tgacactgca aacaccctct
     1081 tctccaattt tgtgtactca gccagcagga gtgccagaaa tcttaaagaa aagcttgcat
     1141 agctgttcag tgaaaggaga agaagaagtg tttttaatcg gcaagaactt tctgaaagga
     1201 actaaagtta ttttccaaga aaatgtttct gatgaaaact cttggaagtc agaagctgaa
     1261 attgatatgg aactatttca tcagaatcat cttattgtga aggttcctcc ctatcatgac
     1321 caacatataa ctttgcctgt gtcagtggga atatatgtag tgacaaatgc tggaagatct
     1381 catgatgttc aaccattcac ttacactcca gacccagcag cagctggtgc tttgaatgta
     1441 aatgtgaaga aggaaatatc tagtccagca agaccttgct cttttgaaga ggccatgaaa
     1501 gcaatgaaaa ctactggatg taatttagat aaggtaaata ttatccctaa tgccctgatg
     1561 actccactca taccaagcag tatgattaag agtgaagatg ttactccaat ggaagtaaca
     1621 gcagaaaaaa gatcttccac tatttttaag actacaaagt ctgttggatc aactcagcaa
     1681 acattagaaa acatctcaaa catagcagga aatggctctt tttcatcacc atcatcttcc
     1741 cacctacctt ctgaaaatga aaaacagcag cagattcagc ccaaggcata caacccagag
     1801 accctgacaa ctattcaaac ccaggacatc tcacagcctg gtacttttcc agcagtttct
     1861 gcttctagtc agctgcccaa cagcgatgca ctattgcagc aggctacaca gtttcagaca
     1921 agagaaactc agtctagaga gatattacag tcagatggta cagtggttaa tttgtcacaa
     1981 ctgactgagg catcacaaca acagcagcag tcaccactac aagaacaagc acagacttta
     2041 cagcagcaga tttcatcaaa tatttttcca tcaccaaata gtgtgagtca gcttcagaat
     2101 actattcagc agctgcaagc agggagtttc acaggcagta ctgctagtgg cagcagtgga
     2161 agtgttgact tggtccaaca agttttagag gcacagcagc agttatcttc agttttattt
     2221 tctgctccag atggtaatga gaatgttcaa gagcagctta gtgcagatat ttttcaacaa
     2281 gtcagtcaaa ttcagagtgg tgtaagccct ggaatgtttt cctcaacaga gccaacagtc
     2341 cataccagac cagataattt attacctgga agagctgaaa gtgttcatcc acagtctgaa
     2401 aacacgttat ctaatcaaca gcagcagcag cagcagcaac agcaagtgat ggaatcttca
     2461 gccgcaatgg tgatggagat gcaacagagt atctgccagg cagctgccca gattcagtca
     2521 gagttattcc cttcaactgc ttcagcaaat ggaaaccttc agcaatcgcc agtttaccag
     2581 cagacttctc acatgatgag tgcattgtct accaatgagg atatgcaaat gcagtgtgaa
     2641 ttgttttctt ctcctcctgc agtttctgga aatgaaactt ctacaactac cacacagcag
     2701 gttgcaaccc ctggcactac catgtttcag acatcaagtt caggagatgg agaagaaact
     2761 ggaacacaag caaaacagat tcagaacagt gtctttcaga ccatggtcca aatgcaacat
     2821 agtggggaca atcaacctca agttaacctt ttttcatcca caaaaagtat gatgagtgtt
     2881 cagaatagtg gtacccaaca acaaggtaat ggtttattcc agcaagggaa tgagatgatg
     2941 tcacttcaat ctggaaattt tttgcagcag tcttctcatt cacaggccca actttttcat
     3001 cctcaaaatc ctattgccga tgctcagaac ctttcccagg aaactcaagg ttctctcttt
     3061 catagtccaa atcctattgt ccacagtcag acttctacaa cctcctctga acaaatgcag
     3121 cctccaatgt ttcactctca aagtaccatt gctgtgttac agggctcttc agttcctcaa
     3181 gaccagcagt caaccaacat atttctttcc cagagtccca tgaataatct tcagactaac
     3241 acagtagccc aagaagcatt ttttgcagca ccgaactcaa tttctccact tcagtcaaca
     3301 tcaaacagtg aacaacaagc tgctttccaa cagcaagctc caatatcaca catccagact
     3361 cctatgcttt cccaagaaca ggcacaaccc ccgcagcagg gtttatttca gcctcaggtg
     3421 gccctgggct cccttccacc taatccaatg cctcaaagcc aacaaggaac catgttccag
     3481 tcacagcact caatagttgc catgcagagt aactctccat cccaggaaca gcagcagcag
     3541 cagcaacagc agcagcaaca gcagcagcaa caacaacaga gcattttatt cagtaatcag
     3601 aataccatgg ctacaatggc gtctccaaag caaccaccac caaacatgat attcaaccca
     3661 aatcaaaatc caatggctaa tcaggagcaa cagaaccagt caatttttca ccaacaaagt
     3721 aacatggccc caatgaatca agagcaacag cccatgcaat ttcagagtca gtccacagtt
     3781 tcctcacttc agaacccagg tcctacccag tcggaatcat cacagacccc cttgttccat
     3841 agctctcctc agattcagtt ggtacaaggg tcacctagtt ctcaagagca gcaagtaact
     3901 ctcttcttat ctccagcatc catgtctgcc ttgcagacca gtataaatca acaagatatg
     3961 caacagtctc ctctttattc ccctcagaac aacatgcctg gaattcaagg agccacatct
     4021 tcgcctcaac cacaggctac tttatttcac aacacagcag gaggcacaat gaaccaactg
     4081 cagaattctc ctggctcatc tcagcagaca tcaggaatgt tcttatttgg cattcaaaat
     4141 aactgtagtc agcttttaac ctctggacca gctacattgc ctgatcagtt gatggccata
     4201 agtcagccag gccaaccaca aaacgagggc cagccacctg tgacaacact tctttctcag
     4261 caaatgccag agaattctcc actggcatcc tctataaaca ccaaccagaa catcgaaaag
     4321 attgatttgc ttgtttcatt gcaaaaccaa gggaacaact tgactggctc cttttagtaa
     4381 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     4441 gacgagtaga catgttcaat caacgcaaa
//
