LOCUS       sequence-278687-        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2099
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttcctc aattatctgc caggagcact gccagatctc gggccaggca
       61 aaaatagaca tcttattagt tggagatgtc actgtgggct acctggctga tactgtacag
      121 aaactatttg caaacatagc agaagtcacc atcaccatca gtgacacgaa ggaggcagca
      181 gcgcttttgg atgattgcat attcaacatg gttctcttga aggtgccttc ttcactaagt
      241 gccgaggagc tggaagccat caagttaatt agatttggca aaaagaaaaa tacacattca
      301 ctgtttgttt ttataatccc tgaaaatttt aaaggttgta tttcagggca tggaatggat
      361 attgctttaa ctgaaccact gacaatggaa aaaatgagta atgtggtaaa atactggaca
      421 acatgtccct caaacactgt taagactgaa aacgcaactg ggcctgaaga acttggattg
      481 cccctgcaga ggtcctacag cgaacacctg ggatattttc ctactgatct atttgcctgc
      541 tctgaatctt taaggaatgg caatgggctt gaattaaatg cttcgttgtc agagttcgag
      601 aaaaacaaaa agatctctct tcttcattca agcaaggaaa aactaagaag ggaaagaatc
      661 aaatattgct gtgagcagct gcgtactctc ttgccgtatg taaaagggag aaagaatgat
      721 gcggcttcag ttcttgaggc aacagttgat tatgtgaaat atatccggga gaaaatctct
      781 ccagccgtta tggcccagat tacagaagca cttcagagca acatgaggtt ttgtaagaaa
      841 caacaaacac ccattgagct gtctctccca ggcactgtca tggcacagcg ggaaaacagt
      901 gtgatgagca cttactcccc tgagagaggg ctccaattcc tgactaatac gtgctggaat
      961 gggtgctcca ctcctgatgc agagagctcc ttggatgaag ctgtgagagt tccatcaagc
     1021 tccgcctcag agaatgctat tggtgatcca tataaaactc acatttccag tgcagcgctg
     1081 tctctgaatt ccttgcatac tgtcagatat tattctaaag tcaccccttc ctacgatgca
     1141 actgctgtaa caaatcagaa catttcaatt catttacctt cagccatgcc cccggtctca
     1201 aagcttctcc ctcggcactg cacttctggg ttgggccaga cgtgcactac acatcccaac
     1261 tgtctgcaac agttttgggc gtattagtaa tgaactagtc cgtaacttga aagtatttcg
     1321 atttcttggc tttatatatc ttgtggaaag gacgaggtat cgagacctta tcacctgcg
//
