LOCUS       sequence-278693-        1769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2076
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agagacacta taatgatgaa gaccctgaga aggaaaagcg aataaaggaa
      901 ttagaattgc tcctaatgtc aaccgagaat gagctaaaag gacagcaggt gctaccattc
      961 ttaaacactt ccagtaacca tgaaaactca gacttggaaa tgccttcttt aacttccacc
     1021 cccctcattg gtcacaaatt gactgttaca acaccatttc atagagacca gactgtgaaa
     1081 actcaaaagg aaaatactgt ttttagaacc ccagctatca aaaggtcaat cttagaaagc
     1141 tctccaagaa ctcctacacc attcaaacat gcacttgcag ctcaagaaat taaatacggt
     1201 cccctgaaga tgctacctca gacaccctct catctagtag aagatctgca ggatgtgatc
     1261 aaacaggaat ctgatgaatc tggaattgtt gctgagtttc aagaaaatgg accaccctta
     1321 ctgaagaaaa tcaaacaaga ggtggaatct ccaactgata aatcaggaaa cttcttctgc
     1381 tcacaccact gggaagggga cagtctgaat acccaactgt tcacgcagac ctcgcctgtg
     1441 gcagatgcac cgaatattct tacaagctcc gttttaatgg caccagcatc agaagatgaa
     1501 gacaatgttc tcaaagcatt tacagtacct aaaaacaggt ccctggcgag ccccttgcag
     1561 ccttgtagca gtacctggga acctgcatcc tgtggaaaga tggaggagca gatgacatct
     1621 tccagtcaag ctcgtaaata cgtgaatgca ttctcagccc ggacgctggt catgtagtaa
     1681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1741 gacgaatcta ctaattgaca gtcgtccca
//
