LOCUS       sequence-278717-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0754
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagctct gtcccccaca tttgccacgt ccacccagga ttccacctgt
       61 ctccaggact cagaatttcc tgtttcttca aaagaccatt cctgtcccca gaatttggac
      121 ctgttcgtgt gcagtggtct ggagcctcac acacccagtg ttggttccca ggaatcggtg
      181 actttccaag atgtcgctgt ggactttacc gagaaggagt ggcccttgct ggattcttct
      241 cagagaaaac tgtacaaaga tgtaatgctg gagaactaca gcaacctcac ttcactggat
      301 tgggagactc catctaaaac caagtggtca cttcttatgg aagatatttt tgggaaggaa
      361 acgcccagtg gtgtgacgat ggaaagagcc ggtcttggag agaagtccac tgaatacgct
      421 cacttgttcg aagtctttgg catggaccct catctcactc agccaatggg aaggcacgct
      481 ggcaagaggc cctatcaccg ccgcgactat ggggtagcgt tcaagggcag gccgcacctc
      541 actcagcaca tgagcatgta cgacgggaga aaaatgcatg aatgtcatca gtgccaaaaa
      601 gccttcacca cgagcgcgtc cctcacacgg cacaggagaa tccacaccgg ggagaagcct
      661 tacgagtgca gcgactgcgg gaaagccttc aacgaccctt cagcccttag gagccacgca
      721 agaactcacc tcaaagagaa gccctttgac tgcagtcagt gtggaaatgc attccggacc
      781 ctctcggccc tgaaaatcca catgcgagtt cacactggcg agaggcctta caagtgtgat
      841 cagtgcggga aggcttacgg ccggagctgc cacctcatcg cacacaagag aacgcacacc
      901 ggagagaggc cctacgagtg tcacgactgt gggaaagctt tccagcaccc ctcccacctc
      961 aaagagcacg tgaggaatca cacgggggag aagccctacg cgtgcacgca gtgcggcaaa
     1021 gccttccgct ggaagtccaa ctttaatttg cacaagaaga accacatggt ggagaagacc
     1081 tacgaatgta aagaatgcgg gaaatccttt ggcgatctcg tgtcccggag gaaacacatg
     1141 aggattcaca tcgtcaagaa acccgtggaa tgtcggcagt gcgggaagac cttccgaaac
     1201 cagtccatcc ttaagactca catgaactct cacactggag agaaaccata cgggtgcgat
     1261 ctctgcggga aagctttcag cgcgagttca aacctcaccg cacacaggaa gatacacacg
     1321 caagagagac gctacgaatg cgccgcctgc gggaaagtct tcggtgacta tttatcccgg
     1381 cggaggcaca tgagcgttca ccttgtaaag aaacgagttg agtgtaggca gtgtggcaag
     1441 gccttcagga accagtcaac gctgaagacg cacatgcgaa gccacacggg ggagaaaccg
     1501 tacgaatgcg atcactgtgg gaaggccttc agcataggct ccaacctgaa tgtgcacagg
     1561 cggatccaca ccggggagaa gccctacgaa tgccttgtct gcgggaaagc cttcagcgac
     1621 cactcatccc tcaggagcca cgtgaaaact caccggggag agaagctctt tgtgtcatcc
     1681 gtgtggaaaa ggctccagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1741 tggctttata tatcttgtgg aaaggacgaa gatagccaga acaaatttcc cga
//
