LOCUS       sequence-278720-        1472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0761
FEATURES             Location/Qualifiers
     source          1..1472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacacagt ggtctttgaa gacgtggttg tggatttcac gctggaggag
       61 tgggccttgc tgaatcctgc tcagagaaaa ctctacagag atgtcatgct ggagaccttc
      121 aagcacctgg cctcagtaga taatgaggct cagcttaaag ccagtgggtc tatttctcag
      181 caggatactt ctggagaaaa attatccctc aaacagaaaa tagaaaagtt cacaagaaag
      241 aatatatggg cctccctttt aggaaaaaat tgggaagaac atagcgttaa agacaagcac
      301 aacaccaagg agagacattt gagcagaaat ccaagggtgg agagaccatg taaaagcagt
      361 aaaggtaata aacgtggaag aaccttcaga aagactcgaa attgtaatcg tcatctgcgc
      421 aagaattgtt gtactagtgt aagacggtac gaatgcagtc agtgtggaaa actcttcacc
      481 cattcctcat ccctgataag gcacaaaaga gctcactctg gacaaaaatt atataaatgt
      541 aaggaatgtg ggaaagcctt cagtcgccct tcctacctac agacgcatga gaaaactcac
      601 agtggagaga aaccctatgc ctgtcaatct tgcgggaaga catttcttcg ttcccactct
      661 ctcactgaac atgtaaggac tcacactgga gagaaaccct acgaatgtgg gcagtgtggg
      721 aaaggcttca gttgtcccaa atcctttcgc gcacatgtga tgatgcacgc cggagggaga
      781 ccgtatgagt gcaagcactg tgggaaagcc ttcaggtgtc agaaatcctt tcgagtccat
      841 atgatcatgc acgccggagg gagaccgtat gagtgcaagc agtgtgggaa agcctactgc
      901 tgggcaacat cctttcaacg acacgtgaga attcacaacg gggagaaacc ctataagtgt
      961 ggaaaatgcg ggaaagcatt cggttggccc tcatccttac acaaacacgc gagaacgcat
     1021 gctaaaaaga aacctgtgag tgggggcagc gtgggaaagt cttccgcgag gcctcgcccc
     1081 tccacagatg tcaaatcaca aactagagag aaagtctata aatgtgaaac gtgtgggaaa
     1141 acgtatggtt ggtcctcatc tttacacaaa catgagagaa agcacactgg ggagaaacct
     1201 gtaaatgcag ccagtgtggg aaaaccttca ggcgggcttt gctcttccaa aaatgtaaga
     1261 acgcagattg gacagaagcc cagtaaatgc gaaaaatgtg ggaaagcttt cagttgtccc
     1321 aaagcctttc aaggtcatgt gagaagtcac acaggaaaga aatcctgtac atctaagtag
     1381 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1441 aaggacgagg aacgacgatg cggtatcacc cg
//
