LOCUS       sequence-278729-        1715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0913
FEATURES             Location/Qualifiers
     source          1..1715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggact cttgagcgtc gccctccagg gcgcagagtt ggaaggaaac
       61 tggaaacacg agggccaggt tgaggacctg caggaaaacc aggaaagctg tccagagcca
      121 gaggccgtgg cctgcaaggg agaccctgct ggggacagca tgcaggaacg cgatgaattc
      181 agccgaatcc caagaaccat atcgagccct gctgctaccc aagccagtgt ccccgacgac
      241 agcagttccc ggaggtgcag cgcgcctggg gagagcccga aggagaggca tcctgacagc
      301 cgccagcggg agagaggtgg aggccccaag aagccgtgga aatgcgggga ctgcgggaag
      361 gccttcagct actgttcagc gttcatctta caccagagaa tccacaccgg ggagaagcca
      421 tttgcgtgcc ccgagtgcgg caaggccttc agccagagcg tgcacctgac cctgcaccag
      481 cgcacgcaca cgggcgagaa gccctacgcc tgccacgagt gcggcaaggc cttcagccag
      541 ggctcgtacc tggcgtccca ctggcgcacg cacacgggcg agaagccgca ccgctgcgcc
      601 gactgcggca aggccttcac gcgcgtcacg cacctgaccc agcaccggcg cgtgcacacg
      661 ggcgagcggc cctacgcgtg cgcccagtgc gccaaggcgt tccgcaaccg ctcgtccctg
      721 atagagcacc agcgcatcca caccggtgag aagccctacg agtgctccgc gtgcgccaag
      781 gccttccgct tctcctcagc gctcatccgc caccagcgca tccacacgga ggagaagccc
      841 taccgctgcg gccagtgcgc caaggccttc gcgcagatcg cgcacctgac ccagcaccgg
      901 cgcgtgcaca cgggcgagaa gccctacacg tgccaggact gcggcgcgct cttcagccag
      961 agcgcctctc tggccgagca ccggcgcatc cacacaggcg agaagcccta cgcgtgcggc
     1021 cagtgcgcca aggccttcac ccaggtgtcg cacctgacgc agcaccagcg cacgcacacc
     1081 ggagagcggc cttacccctg ccacgactgc ggcaagcgct tcagcaaccg ctcccacctc
     1141 ctccagcacc gcctcgtgca caccggtgag cggccctaca ggtgcctgca atgcggggcc
     1201 gccttcagcc acgtgtcctc gcttatagag caccagaaga tccacaccgg agagcggccc
     1261 tacaagtgcg gcgagtgcgg caaagctttc agccagggct cctcgctcgc cctgcaccag
     1321 cgcacgcaca cgggcgagcg gccctacacc tgccccgagt gcggcaaggc cttcagcaac
     1381 cgctcctacc tgatccagca ccacatcgtg cacaccgggg agaagcccta cgagtgcagc
     1441 ggctgcggga aggccttcag cttctcctcc gcgctcatcc gacaccagag gacgcatgca
     1501 gacagttcgg gacgcctttg cccagctccc acgcctgact caacacctgg gctctcacag
     1561 ggaggagaaa cctgtcaaca ggggtgccct ggcagaaacc cgcgtggacc agcagaagat
     1621 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1681 ggaaaggacg atctagaaag ccctaagtcc cacct
//
