LOCUS       sequence-278747-        1985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0764
FEATURES             Location/Qualifiers
     source          1..1985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactcagt ggtctttgag gatgtggctg tggacttcac cctggaggag
       61 tgggctttgc tggattctgc tcagagggac ctctacagag atgtgatgct ggagaccttt
      121 cagaacctgg cctcagtaga tgatgaaact caatttaagg ccagtgggtc agtttctcag
      181 caggatattt atggagagaa aatacccaag gaatctaaaa tagccacgtt caccagaaat
      241 gtttcctggg cctctgtttt aggaaaaatt tgggacagtc ttagcatcga agatcaaacc
      301 acaaaccagg ggagaaatct cagaaatcat gggttggaga gactctgtga aagtaatgat
      361 caatgtggag aagccctcag ccagattcca catcttaatc tgtacaagaa aattccacct
      421 ggagtaaaac agtatgaata caacacgtac ggaaaagtct tcatgcatcg ccgcacatcc
      481 ctcaagagtc ccatcacagt tcacactgga cacaaaccat atcagtgcca ggaatgtggg
      541 caggcctaca gttgtcgttc acacctaaga atgcatgtga gaacccacaa tggagagaga
      601 ccctatgtgt gtaaattatg tgggaaaacc tttcctcgta cttcctccct caatcggcat
      661 gtaaggattc acactgctga gaaaacctac gaatgtaagc aatgtgggaa agcctttatt
      721 gacttctcaa gtcttactag tcatctcaga agtcacaccg gagagaagcc atataagtgt
      781 aaggaatgtg ggaaagcttt cagttattcc tcaacgtttc gaagacacac aataacacac
      841 actggcgaga agccatataa atgtaaggaa tgtgcggaag cctttagtta ttcctcaact
      901 tttcgaagac atatgatttc acacactgga gagaagccac ataaatgtaa agaatgtggg
      961 gaggccttca gttattcttc ggcttttcga agacacatga taacacacac tggagagaaa
     1021 ccctacgaat gcaaacaatg tgggaaaacc ttcatttatc tccagtcctt tcgaagacat
     1081 gaaaggattc acactggaga gaaaccctac gaatgcaaac agtgtgggaa gaccttcatt
     1141 tatccccagt cctttcgaag acatgaaagg actcatggtg gagagaaacc ctatgaatgc
     1201 aaccagtgcg ggaaagcatt cagtcacccc tcctcctttc gaggacacat gagggtgcac
     1261 actggagaga aaccctatga gtgcaagcaa tgtgggaaaa ctttcaattg gcccatatct
     1321 ttacgaaaac atatgagaac acatactaga gagaaaccct atgaatgtaa gcagtgtggg
     1381 aaagccttca gcttgtctgc ttgctttcga gaacatgtga gaatgcaccc tgaagacaaa
     1441 tcctatgaat gcaagctatg tgggaaagct ttctattgcc acatatcctt acaaaaacat
     1501 atgagaagac ataccgcaga gaaactctat aaatgcaagc agtgtgggaa agctttcagt
     1561 tggcctgaac ttttgcaaca acatgtgaga acgcacactg tagagaagcc ctatgaatgt
     1621 aaggaatgtg ggaaggtctt caaatggcca tcatctttac caatacatat gagactgcac
     1681 actggagaga aaccttatca atgtaagcat tgtgggaaag cattcaattg ttcctcatcc
     1741 ttaaggcgac atgtgagaat acacactaca gaaaaacagt ataagtgtaa tgtaggacat
     1801 cctcctgcaa atgaattcat gtgcagtgct tcagaaaagt cacaccagga gagagatctg
     1861 atcaaagttg taaatatggt gttgccttta tagtaatgaa ctagtccgta acttgaaagt
     1921 atttcgattt cttggcttta tatatcttgt ggaaaggacg aacctggctc cactacggct
     1981 ctccc
//
