LOCUS       sequence-27875-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of ChR2-GFP and Halo-YFP for bi-directional control of 
            neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Han X, Qian X, Stern P, Chuong AS, Boyden ES
  TITLE     Informational lesions: optical perturbation of spike timing and 
            neural synchrony via microbial opsin gene fusions.
  JOURNAL   Front Mol Neurosci. 2009 Aug 27:2:12. doi: 10.3389/neuro.02.012.2009
  PUBMED    19753326
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci. 2009 Aug 27:2:12. doi: 10.3389/neuro.02.012.2009"
COMMENT     Sequence Label: ChR2-2A-Halo
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(185..436)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(227..248)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     576..595
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     835..856
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctacaaatgt ggtatggctg attatgatct agagtcgcgg ccgctcaaca tgtgaattct
       61 ctagaactga gaccctccca cccgtgactg aaagcgccgt cgctctgcaa gcagaggtta
      121 cccagcggga gctgttcgag ttcgtcctca acgaccccct cctggcttct agccggaatt
      181 cttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      241 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt
      301 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      361 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      421 cgttcttctg cttgtcggcc atgatataga cgttgtgtct gttgtagttg tactccagct
      481 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      541 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      601 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      661 gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagccgaag gtggtcacga
      721 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      781 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      841 cgtccagctc gaccag
//
