LOCUS       sequence-27876-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of ChR2-GFP and Halo-YFP for bi-directional control of 
            neuronal activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Han X, Qian X, Stern P, Chuong AS, Boyden ES
  TITLE     Informational lesions: optical perturbation of spike timing and 
            neural synchrony via microbial opsin gene fusions.
  JOURNAL   Front Mol Neurosci. 2009 Aug 27:2:12. doi: 10.3389/neuro.02.012.2009
  PUBMED    19753326
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci. 2009 Aug 27:2:12. doi: 10.3389/neuro.02.012.2009"
COMMENT     Sequence Label: ChR2-2A-Halo
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    117..238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(128..147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     182..201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(500..751)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(542..563)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
       61 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt acgccttaag
      121 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      181 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      241 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      301 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
      361 caacatgtga attctctaga actgagaccc tcccacccgt gactgaaagc gccgtcgctc
      421 tgcaagcaga ggttacccag cgggagctgt tcgagttcgt cctcaacgac cccctcctgg
      481 cttctagccg gaattcttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
      541 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      601 actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      661 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
      721 agttcacctt gatgccgttc ttctgcttgt cggccatgat ata
//
