LOCUS       sequence-278762-        2120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0831
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(575..592)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tggagagtag aaaactgatt tctgctacag acattcagta ctctggcagt
       61 ctgctgaact ccttgaatga gcaacgtggc catggactct tctgtgatgt taccgttatt
      121 gtggaagacc gaaaattccg ggctcacaag aatattcttt cagcttctag tacctacttc
      181 catcagctct tctctgttgc tgggcaagtt gttgaactga gctttataag agcagagatc
      241 tttgcagaaa ttctcaatta tatctatagt tctaaaattg ttcgtgttag atcagatttg
      301 cttgatgagt taattaaatc agggcagtta ttaggagtga aatttatagc agagcttggt
      361 gtcccattgt cacaggttaa aagcatctca ggtacagcgc aggatggtaa tactgagcct
      421 ttacctcctg attctggtga caagaacctt gtaatacaga aatcaaaaga tgaagcccaa
      481 gataatgggg ctactataat gcctattata acagagtctt tttcattatc tgccgaagat
      541 tatgaaatga aaaagatcat tgttaccgat tctgatgatg atgatgatga tgtcattttt
      601 tgctccgaga ttctgcccac aaaggagact ttgccgagta ataacacagt ggcacaggtc
      661 caatctaacc caggccctgt tgctatttca gatgttgcac ctagtgctag caataactcg
      721 ccccctttaa caaatatcac acctactcag aaacttccta ctcctgtgaa tcaggcaact
      781 ttgagccaaa cacaaggaag tgaaaaattg ttggtatctt cagctccaac acatctgact
      841 cccaatatta ttttgttaaa tcagacacca ctttctacac caccaaatgt cagttcttca
      901 cttccaaatc atatgccctc ttcaatcaat ttacttgtgc agaatcagca gacaccaaac
      961 agtgctattt taacaggaaa caaggccaat gaagaggagg aggaggaaat aatagatgat
     1021 gatgatgaca ctattagctc cagtcctgac tcggccgtca gtaatacatc tttggtccca
     1081 caggctgata cctcccaaaa taccagtttt gatggatcat taatacagaa gatgcagatt
     1141 cctacacttc ttcaagaacc actttccaat tccttaaaaa tttcagatat aattactaga
     1201 aatactaatg atccaggcgt aggatcaaaa catctaatgg agggtcagaa gatcattact
     1261 ttagatacag ctactgaaat tgaaggctta tcgactggtt gcaaggttta tgcaaatatc
     1321 ggtgaagata cttatgatat agtgatccct gtcaaagatg accctgatga aggggaggcc
     1381 agacttgaga atgaaatacc aaaaacgtct ggcagcgaga tggcaaacaa acgtatgaaa
     1441 gtaaaacatg atgatcacta tgagttaata gtagatggaa gggtctatta tatctgtatt
     1501 gtatgcaaaa ggtcatatgt ctgtctgaca agcttgcgga gacattttaa cattcattct
     1561 tgggagaaga agtatccgtg ccgttactgt gagaaggtat ttcctcttgc agaatatcgc
     1621 acaaagcatg aaattcatca cacaggggag cgaaggtatc agtgtttggc ctgtggcaaa
     1681 tctttcatca actatcagtt tatgtcttca catataaagt cagttcatag tcaagatcct
     1741 tctggggact caaagcttta tcgtttacat ccatgcaggt ctttacaaat cagacaatat
     1801 gcatatcttt ccgatagatc aagcactatt cctgcaatga aggatgatgg tattgggtat
     1861 aaggttgaca ctggaaaaga acctccagta gggaccacta catctactca gaacaagcca
     1921 atgacctggg aagatatttt tattcagcag gaaaatgatt caatttttaa acaaaatgta
     1981 acagatggca gtactgagtt tgaatttata ataccagagt cttactagta atgaactagt
     2041 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagaag
     2101 atactctttg gacatcctgc
//
