LOCUS       sequence-278771-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0891
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcggg gatgtatttg gaacattatc tggacagtat tgaaaacctt
       61 ccctttgaat tacagagaaa ctttcagctc atgagggacc tagaccaaag aacagaggac
      121 ctgaaggctg aaattgacaa gttggccact gagtatatga gtagtgcccg cagcctgagc
      181 tccgaggaaa aattggccct tctcaaacag atccaggaag cctatggcaa gtgcaaggaa
      241 tttggtgacg acaaggtgca gcttgccatg cagacctatg agatggtgga caaacacatt
      301 cggcggctgg acacagacct ggcccgtttt gaggctgatc tcaaggagaa acagattgag
      361 tcaagtgact atgacagctc ttccagcaaa ggcaaaaaga aaggccggac tcaaaaggag
      421 aagaaagctg ctcgtgctcg ttccaaaggg aaaaactcgg atgaagaagc ccccaagact
      481 gcccagaaga agttaaagct cgtgcgcaca agtcctgagt atgggatgcc ctcagtgacc
      541 tttggcagtg tccacccctc tgatgtgttg gatatgcctg tggatcccaa cgaacccacc
      601 tattgccttt gtcaccaggt ctcctatgga gagatgattg gctgtgacaa ccctgattgt
      661 tccattgagt ggttccattt tgcctgtgtg gggctgacaa ccaagcctcg ggggaaatgg
      721 ttttgcccac gctgctccca agaacggaag aagaaatagt aatgaactag tccgtaactt
      781 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacag ggtcacggac
      841 tacgagtcat c
//
