LOCUS       sequence-278774-        2681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0939
FEATURES             Location/Qualifiers
     source          1..2681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacttcac cttgggagac tgggagcagc tcgggctgga acagggggac
       61 acgttctggg acacagcgtt ggacaattgc caggacctct tcctgctgga ccccccaaga
      121 cccaacctga cctcccaccc agatggcagt gaagatctgg agcctctggc aggaggaagc
      181 ccagaagcaa caagccctga tgtgactgag accaagaact ctcctctgat ggaggatttc
      241 ttcgaagaag gattctccca ggagattata gagatgttat ccaaggatgg cttctggaac
      301 tccaatttcg gagaagcctg tatagaggac acctggttag atagtttgct aggcgatcca
      361 gaaagtcttc tgaggtctga tattgccacc aacggggaaa gtcccacgga atgcaagagt
      421 catgaattaa agagaggact cagtcctgtg tccaccgttt ccacgggaga agattccatg
      481 gtgcataatg tttctgaaaa gaccctcaca ccagctaagt ctaaggaata taggggtgag
      541 tttttctcct actccgacca cagccagcag gattctgttc aggaagggga gaaaccatat
      601 caatgtagtg aatgtgggaa aagcttcagt gggagttacc gtcttaccca gcactggatc
      661 actcatacta gggagaaacc cactgtccat caagagtgtg agcaaggttt tgaccggaat
      721 gcttcccttt ctgtgtatcc gaaaactcac acgggctaca aattctatgt gtgtaatgaa
      781 tatgggacaa cttttagtca gagtacatac ctgtggcatc agaaaactca cactggagaa
      841 aaaccatgta agagtcaaga tagtgaccac ccacccagtc atgacacaca gcctggtgag
      901 catcagaaaa ctcacacaga tagtaagtcc tacaactgta acgaatgcgg caaggctttt
      961 acccggatct tccaccttac tcggcaccag aagatccaca ctcggaaacg ctatgagtgt
     1021 tccaagtgcc aggcgacctt caacttgaga aaacacctca tccaacatca gaaaactcac
     1081 gctgcaaaaa ctacctctga gtgtcaggag tgtgggaaga tttttaggca cagttcgctg
     1141 ctcattgaac accaggctct tcatgctgga gaggagcctt ataagtgtaa cgaacgtggg
     1201 aaatccttca ggcataactc taccctaaag atccatcaga gggttcacag tggagagaag
     1261 ccttacaaat gcagtgagtg tgggaaggcc ttccaccggc acactcacct taatgaacat
     1321 cggcgaattc atacaggcta cagaccccac aaatgtcagg aatgcgtcag gagtttcagc
     1381 cggccctcac atctgatgcg acatcaggcc attcacaccg cagaaaagcc ctatagctgt
     1441 gctgaatgca aggagacttt cagcgataac aatcgccttg tgcaacacca gaaaatgcac
     1501 actgtcaaaa ccccatatga atgtcaggag tgcggagaac gcttcatttg cggctcaacc
     1561 ctgaagtgcc acgagagtgt tcacgccaga gaaaaacaag gattttttgt gagtgggaag
     1621 atcttggatc agaacccaga acagaaagag aagtgcttta agtgtaacaa atgtgagaaa
     1681 acctttagct gcagcaaata cctaactcag cacgagagga ttcacaccag gggagtgaag
     1741 ccctttgaat gtgaccagtg tgggaaagcc tttggccaaa gtactcggct cattcaccat
     1801 caaagaatcc actctagagt gaggctgtat aaatggggtg agcaagggaa agccatcagc
     1861 agtgcctccc ttatcaaact tcagtccttc cacacaaagg agcacccttt taaatgtaac
     1921 gaatgcggaa agaccttcag ccacagtgca cacctctcaa aacatcagtt aattcacgct
     1981 ggagagaatc cctttaaatg tagtaagtgt gacagagtct tcacccagag aaactacctt
     2041 gttcagcatg agcgaactca tgccagaaag aagccgttgg tgtgtaacga atgcgggaaa
     2101 acgttccgtc agagctcatg cctttctaag catcagagaa ttcactcagg tgagaagccc
     2161 tatgtatgtg attactgcgg gaaggccttc ggcctgagtg ctgagcttgt ccgccaccag
     2221 agaattcaca ctggagaaaa gccttatgtt tgtcaggaat gcgggaaagc cttcacccag
     2281 agctcatgcc tttctattca ccggagagtt cacactgggg agaagcccta cagatgtggt
     2341 gaatgtggga aagcctttgc ccagaaagca aatctaacac agcaccagag aattcacaca
     2401 ggggagaagc cttactcctg taatgtgtgt ggcaaagctt ttgtcctcag tgcccatctc
     2461 aaccagcacc tgagagttca cacccaggag acactttatc agtgtcaacg ttgccagaaa
     2521 gcctttcggt gccactcgag cctcagccgc catcagcgtg tacacaacaa gcagcaatac
     2581 tgcctgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2641 tcttgtggaa aggacgaatc gagaggtcag acacatttac c
//
