LOCUS       sequence-278810-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0667
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(160..177)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 accgccacca tggaggacct ggatgccctg ctctctgacc tggagactac cacctcgcac
       61 atgccaaggt caggggctcc caaagagcgc cctgcggagc ctctcacccc tcccccatcc
      121 tatggccacc agccacagac agggtctggg gagtcttcag gagcctcggg ggacaaggac
      181 cacctgtaca gcacggtatg caagcctcgg tccccaaagc ctgcagcccc ggcggcccct
      241 ccattctcct cttccagcgg tgtcttgggt accgggctct gtgagctaga tcggttgctt
      301 caggaactta atgccactca gttcaacatc acagatgaaa tcatgtctca gttcccatct
      361 agcaaggtgg cttcaggaga gcagaaggag gaccagtctg aagataagaa aagacccagc
      421 ctcccttcca gcccgtctcc tggcctccca aaggcttctg ccacctcagc cactctggag
      481 ctggatagac tgatggcctc actctctgac ttccgcgttc aaaaccatct tccagcctct
      541 gggccaactc agccaccggt ggtgagctcc acaaatgagg gctccccatc cccaccagag
      601 ccgactggca agggcagcct agacaccatg ctggggctgc tgcagtccga cctcagccgc
      661 cggggtgttc ccacccaggc caaaggcctc tgtggctcct gcaataaacc tattgctggg
      721 caagtggtga cggctctggg ccgcgcctgg caccccgagc acttcgtttg cggaggctgt
      781 tccaccgccc tgggaggcag cagcttcttc gagaaggatg gagccccctt ctgccccgag
      841 tgctactttg agcgcttctc gccaagatgt ggcttctgca accagcccat ccgacacaag
      901 atggtgaccg ccttgggcac tcactggcac ccagagcatt tctgctgcgt cagttgcggg
      961 gagcccttcg gagatgaggg tttccacgag cgcgagggcc gcccctactg ccgccgggac
     1021 ttcctgcagc tgttcgcccc gcgctgccag ggctgccagg gccccatcct ggataactac
     1081 atctcggcgc tcagcgcgct ctggcacccg gactgtttcg tctgcaggga atgcttcgcg
     1141 cccttctcgg gaggcagctt tttcgagcac gagggccgcc cgttgtgcga gaaccacttc
     1201 cacgcacgac gcggctcgct gtgcgccacg tgtggcctcc ctgtgaccgg ccgctgcgtg
     1261 tcggccctgg gtcgccgctt ccacccggac cacttcacat gcaccttctg cctgcgcccg
     1321 ctcaccaagg ggtccttcca ggagcgcgcc ggcaagccct actgccagcc ctgcttcctg
     1381 aagctcttcg gctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgatcgcgca cccatgcaat gaagaat
//
