LOCUS       sequence-278816-        3011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0723
FEATURES             Location/Qualifiers
     source          1..3011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaaga tgatccatat ttgggaaggc ctgaacaaat gtttcatttg
       61 gatccttctt tgactcatac aatatttaat ccagaagtat ttcaaccaca gatggcactg
      121 ccaacagcag atggcccata ccttcaaata ttagagcaac ctaaacagag aggatttcgt
      181 ttccgttatg tatgtgaagg cccatcccat ggtggactac ctggtgcctc tagtgaaaag
      241 aacaagaagt cttaccctca ggtcaaaatc tgcaactatg tgggaccagc aaaggttatt
      301 gttcagttgg tcacaaatgg aaaaaatatc cacctgcatg cccacagcct ggtgggaaaa
      361 cactgtgagg atgggatctg cactgtaact gctggaccca aggacatggt ggtcggcttc
      421 gcaaacctgg gtatacttca tgtgacaaag aaaaaagtat ttgaaacact ggaagcacga
      481 atgacagagg cgtgtataag gggctataat cctggactct tggtgcaccc tgaccttgcc
      541 tatttgcaag cagaaggtgg aggggaccgg cagctgggag atcgggaaaa agagctaatc
      601 cgccaagcag ctctgcagca gaccaaggag atggacctca gcgtggtgcg gctcatgttt
      661 acagcttttc ttccggatag cactggcagc ttcacaaggc gcctggaacc cgtggtatca
      721 gacgccatct atgacagtaa agcccccaat gcatccaact tgaaaattgt aagaatggac
      781 aggacagctg gatgtgtgac tggaggggag gaaatttatc ttctttgtga caaagttcag
      841 aaagatgaca tccagattcg attttatgaa gaggaagaaa atggtggagt ctgggaagga
      901 tttggagatt tttcccccac agatgttcat agacaatttg ccattgtctt caaaactcca
      961 aagtataaag atattaatat tacaaaacca gcctctgtgt ttgtccagct tcggaggaaa
     1021 tctgacttgg aaactagtga accaaaacct ttcctctact atcctgaaat caaagataaa
     1081 gaagaagtgc agaggaaacg tcagaagctc atgcccaatt tttcggatag tttcggcggt
     1141 ggtagtggtg ctggagctgg aggcggaggc atgtttggta gtggcggtgg aggagggggc
     1201 actggaagta caggtccagg gtatagcttc ccacactatg gatttcctac ttatggtggg
     1261 attactttcc atcctggaac tactaaatct aatgctggga tgaagcatgg aaccatggac
     1321 actgaatcta aaaaggaccc tgaaggttgt gacaaaagtg atgacaaaaa cactgtaaac
     1381 ctctttggga aagttattga aaccacagag caagatcagg agcccagcga ggccaccgtt
     1441 gggaatggtg aggtcactct aacgtatgca acaggaacaa aagaagagag tgctggagtt
     1501 caggataacc tctttctaga gaaggctatg cagcttgcaa agaggcatgc caatgccctt
     1561 ttcgactacg cggtgacagg agacgtgaag atgctgctgg ccgtccagcg ccatctcact
     1621 gctgtgcagg atgagaatgg ggacagtgtc ttacacttag caatcatcca ccttcattct
     1681 caacttgtga gggatctact agaagtcaca tctggtttga tttctgatga cattatcaac
     1741 atgagaaatg atctgtacca gacgcccttg cacttggcag tgatcactaa gcaggaagat
     1801 gtggtggagg atttgctgag ggctggggcc gacctgagcc ttctggaccg cttgggtaac
     1861 tctgttttgc acctagctgc caaagaagga catgataaag ttctcagtat cttactcaag
     1921 cacaaaaagg cagcactact tcttgaccac cccaacgggg acggtctgaa tgccattcat
     1981 ctagccatga tgagcaatag cctgccatgt ttgctgctgc tggtggccgc tggggctgac
     2041 gtcaatgctc aggagcagaa gtccgggcgc acagcactgc acctggctgt ggagcacgac
     2101 aacatctcat tggcaggctg cctgctcctg gagggtgatg cccatgtgga cagtactacc
     2161 tacgatggaa ccacacccct gcatatagca gctgggagag ggtccaccag gctggcagct
     2221 cttctcaaag cagcaggagc agatcccctg gtggagaact ttgagcctct ctatgacctg
     2281 gatgactctt gggaaaatgc aggagaggat gaaggagttg tgcctggaac cacgcctcta
     2341 gatatggcca ccagctggca ggtatttgac atattaaatg ggaaaccata tgagccagag
     2401 tttacatctg atgatttact agcacaagga gacatgaaac agctggctga agatgtgaag
     2461 ctgcagctgt ataagttact agaaattcct gatccagaca aaaactgggc tactctggcg
     2521 cagaaattag gtctggggat acttaataat gccttccggc tgagtcctgc tccttccaaa
     2581 acacttatgg acaactatga ggtctctggg ggtacagtca gagagctggt ggaggccctg
     2641 agacaaatgg gctacaccga agcaattgaa gtgatccagg cagcctccag cccagtgaag
     2701 accacctctc aggcccactc gctgcctctc tcgcctgcct ccacaaggca gcaaatagac
     2761 gagctccgag acagtgacag tgtctgcgac agcggcgtgg agacatcctt ccgcaaactc
     2821 agctttaccg agtctctgac cagtggtgcc tcactgctaa ctctcaacaa aatgccccat
     2881 gattatgggc aggaaggacc tctagaaggc aaaatttagt aatgaactag tccgtaactt
     2941 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaact cgcagtactg
     3001 ggtgtaacct a
//
