LOCUS       sequence-278822-        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0812
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaaccct ctcgtcagag gagagctgcg atagttttga ctcactagag
       61 tcagggaaac agcaggatgt gcgctttcat tccaaatact tcacagaaga gctaagaaga
      121 atttttatag aggacactga ctcagagact gaggattttg caggatttac gcagagtgat
      181 ctgaatggaa agactaaccc agaagtaatg gtcgtggagt cagatttgag tgatgatggc
      241 aaagcatctt tggtgagcga ggaagaggaa gatgaagaag aagataaggc tacccctaga
      301 agaagcaggt ctagaagaag tagtattggt cttcgagtag cctttcagtt ccccaccaag
      361 aagctggcca acaaaccaga taaaaacagt tcttccgagc agttgttttc tagcgcacgc
      421 ttacagaatg agaaaaaaac aattcttgaa agaaagaaag actgtagaca ggtgatacaa
      481 agggaagatt ctacctctga gtctgaggat gactctcggg atgagagcca ggagagttca
      541 gatgctttgc tgaaaaggac catgaacatc aaggagaaca aagccatgct tgcccagtta
      601 ttggcggaat tgaactcgat gccagatttc ttcccagtac gaaccccaac ctcagcttct
      661 aggaagaaga cagtgaggcg ggccttctcg gagggacaga tcacgcggcg tatgaaccca
      721 acccggagtg cgcggcctcc tgagaagttt gctctagaga acttcactgt ctcagccgct
      781 aaatttgcgg aagagtttta cagcttccga agaaggaaga caattggggg gaaatgccgg
      841 gagtacagac gacgtcaccg tatatcttct tttcggccag tggaggatat caccgaagag
      901 gacttagaaa atgttgccat aactgttcga gataaaatct atgataaagt tctgggtaac
      961 acgtgccatc agtgtcgaca aaagaccatc gacaccaaga cagtgtgtcg gaaccagggt
     1021 tgctgtggtg tgcgaggaca gttctgtgga ccatgcctgc ggaaccgcta tggggaggat
     1081 gtcagatcgg cattgctgga cccggattgg gtgtgtcccc cctgtcgtgg gatctgcaat
     1141 tgcagctact gtcggaagcg tgacggccgc tgtgccacag gaatcctcat tcatctggcc
     1201 aagttttatg gttatgacaa tgttaaggaa tatctggaga gcttacaaaa ggagctggta
     1261 gaagacaatt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1321 atatcttgtg gaaaggacga ttccaatcgc ccattcacct ttac
//
