LOCUS       sequence-278837-        1487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0713
FEATURES             Location/Qualifiers
     source          1..1487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggattcagt ggcctttgag gatgtggctg tgaacttcac cctggaggag
       61 tgggctttgc tggatccttc ccagaagaat ctctacaggg atgtgatgcg ggaaaccttc
      121 aggaacctgg cttctgtagg aaaacaatgg gaagaccaga acattgaaga cccattcaaa
      181 attcccagga gaaatataag tcatattcca gagagactct gtgaaagtaa agaaggtggt
      241 caaggtgaag aaaccttcag ccagattcca gatggtattc tgaacaagaa aactcctgga
      301 gtaaaaccgt gtgaaagcag tgtgtgtgga gaagttggca tgggtccttc atcacttaat
      361 aggcacatca gagatcacac tggacgtgaa ccaaatgaat atcaggaata tggaaagaag
      421 tcatatacac gtaaccagtg tggacgagcc ttgagttatc atcgctcttt tccagtacgt
      481 gaaaggactc atcctggagg aaagccctat gattgtaagg aatgtggaga aacctttatt
      541 tctcttgtaa gcattcgaag acacatgtta acgcataggg gaggtgtacc ttacaaatgt
      601 aaggtgtgtg ggaaagcctt tgattatccc agtttatttc gtatacatga aagaagtcac
      661 actggagaga aaccttatga atgcaagcaa tgtgggaaag ccttcagttg ttccagttac
      721 attagaatac atgaaaggac tcacactgga gataaaccct atgaatgcaa gcagtgtggg
      781 aaagctttca gttgttccaa gtacattcga atccatgaac gaactcacac aggagagaaa
      841 ccctacgaat gtaaacagtg cggtaaagcc tttaggtgcg ccagttctgt tcgaagtcac
      901 gagaggactc acaccggaga gaaacttttt gaatgtaagg aatgcgggaa ggctttgact
      961 tgtcttgcaa gtgttagaag acacatgata aagcacactg gcaatggacc ttataaatgt
     1021 aaggtgtgtg ggaaagcctt tgatttcccc agttcatttc gaatccatga aaggacccac
     1081 actggagaga aaccctatga ttgtaagcaa tgtgggaaag ccttcagttg ttccagttcg
     1141 tttcgaaaac atgaaagaat tcacactgga gagaaaccct ataaatgtac aaaatgtggg
     1201 aaagccttca gtcgttccag ttacttccga atccatgaaa gaactcacac tggagagaaa
     1261 ccctatgaat gtaagcaatg tgggaaagcc ttcagtcgat ccacttactt tcgagtacat
     1321 gaaaaaattc atactggaga gaaaccctat gagaacccta accctaacgc ttcagttgtc
     1381 ccagttcttt catagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1441 tatatatctt gtggaaagga cgaaggaagt gcttatgaac gatttac
//
