LOCUS       sequence-278846-        1871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0750
FEATURES             Location/Qualifiers
     source          1..1871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggctga agagacgatg gaatgccttc aggagttccc tgaacatcat
       61 aaaatgatcc tcgaccgatt gaatgaacag cgagagcagg accggtttac tgacatcacc
      121 ctaattgtcg acggacacca ttttaaggct cacaaggctg ttttggctgc ttgtagtaag
      181 ttcttctaca aattctttca ggagtttacc caagaaccat tggtggagat agaaggtgtt
      241 agtaaaatgg cctttcgcca tttaattgag ttcacatata cagcaaaatt aatgatacaa
      301 ggagaagaag aagccaatga tgtatggaaa gcagcagagt ttctacaaat gctagaagct
      361 atcaaagccc ttgaagtcag gaacaaagaa aactcagctc ccttagagga aaataccaca
      421 ggaaaaaatg aggccaaaaa aaggaagatt gcagaaactt caaatgttat cactgagtca
      481 ttgccatctg cagaatcaga acctgttgaa attgaggtag agattgccga aggcaccatt
      541 gaagtggaag atgaaggcat cgaaacatta gaggaagtgg cttctgccaa gcagtccgta
      601 aagtacatac agagcacagg ttcctctgat gattctgctc tagcactgtt ggcagatatt
      661 accagcaagt accgtcaagg tgacagaaaa gggcagatta aagaagatgg ctgtccatct
      721 gaccccacga gcaaacagga gcacatgaaa tcacactcca ctgagagttt caagtgtgaa
      781 atatgcaata aacgatatct tcgagagagc gcatggaaac agcacctaaa ttgttaccac
      841 cttgaagaag gtggagtcag taagaagcaa agaactggga aaaaaattca tgtatgtcag
      901 tactgtgaga aacagtttga ccattttgga cattttaaag aacatcttcg aaaacataca
      961 ggtgaaaaac cttttgaatg tccaaattgt catgaacgat ttgctagaaa tagcactctg
     1021 aaatgtcacc tcactgcatg ccaaactgga gtaggggcaa aaaaaggaag gaagaagctc
     1081 tacgaatgcc aggtctgcaa cagtgtgttt aacagctggg accagttcaa agatcacttg
     1141 gtaatacaca ctggagataa acccaaccat tgtactttat gtgatttgtg gtttatgcaa
     1201 ggaaatgaat taaggaggca tctcagtgat gctcacaata tttcagagcg tctagtaacg
     1261 gaagaagttc tttcagtaga aacacgtgtg caaactgaac ctgtaacatc aatgactatt
     1321 atagaacaag ttgggaaggt gcatgtgcta ccattgcttc aggttcaggt ggattcagca
     1381 caagtgactg tggaacaagt ccatccagat ctgctccagg acagccaggt gcacgattca
     1441 cacatgagtg agcttccaga gcaggtccaa gtgagttatc tagaagtggg ccgaattcag
     1501 actgaagaag gtactgaagt acatgtagag gagctgcatg ttgaacgggt caatcaaatg
     1561 ccagtggaag tacaaactga acttctagaa gcagatttgg accacgtgac cccagaaatc
     1621 atgaaccaag aggagagaga gtctagccaa gcagatgctg ctgaggctgc cagggaagat
     1681 cacgaagatg ctgaggattt agagaccaag ccaacagtgg attctgaagc agaaaaggca
     1741 gagaatgagg acagaacagc tctgccagtt ttagaatagt aatgaactag tccgtaactt
     1801 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagac cgagtcaaca
     1861 ggttccgtaa a
//
