LOCUS       sequence-278855-        1553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0794
FEATURES             Location/Qualifiers
     source          1..1553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagacga cagaccgttt gtgtgcaatg ccccgggctg tggacagaga
       61 tttacaaacg aggaccacct ggcagttcat aaacacaagc atgagatgac attgaaattt
      121 ggcccagccc gaactgactc agtcatcatt gcagatcaaa cgcctactcc aactagattc
      181 ctgaagaact gtgaggaggt gggactcttc aatgaactag ctagctcctt tgaacatgaa
      241 ttcaagaaag ctgcagatga ggatgagaaa aaggctgctg ctgggcccct tgacatgtct
      301 ctgccttcca caccagacat caaaatcaaa gaagaagagc cagtggaggt agactcatcc
      361 ccacctgata gccctgcctc tagtccctgt tccccaccac tgaaggagaa ggaggttacc
      421 ccaaagcctg ttctgatctc tacccccaca cccaccattg tacgtcctgg ctccctgcct
      481 ctccacttgg gctatgatcc acttcatcca acccttccct ccccaacctc tgtcatcaca
      541 caggctccac catccaacag gcaaatgggg tctcccactg gctccctccc tcttgtcatg
      601 catcttgcta atggacagac catgcctgtg ttgccagggc ctccagtaca gatgccgtct
      661 gttatatcgc tggccagacc tgtgtccatg gtgcccaaca ttcctggtat ccctggccca
      721 ccagttaaca gtagtggctc catttctccc tctggccacc ctataccatc agaagccaag
      781 atgagactga aagccaccct aactcaccaa gtctcctcaa tcaatggtgg ttgtggaatg
      841 gtggtgggta ctgccagcac catggtgaca gcccgcccag agcagagcca gattctcatc
      901 cagcaccctg atgccccatc ccctgcccag ccacaggtct caccagctca gcccacccct
      961 agtactgggg ggcgacggcg gcgcacagta gatgaagatc cagatgagcg acggcagcgc
     1021 tttctggagc gcaaccgggc tgcagcctcc cgctgccgcc aaaagcgaaa gctgtgggtg
     1081 tcctccctag agaagaaggc cgaagaactc acttctcaga acattcagct gagtaatgaa
     1141 gtcacattac tacgcaatga ggtggcccag ttgaaacagc tactgttagc tcataaagac
     1201 tgcccagtca ctgcactaca gaaaaagact caaggctatt tagaaagccc caaggaaagc
     1261 tcagagccaa cgggttctcc agcccctgtg attcagcaca gctcagcaac agcccctagc
     1321 aatggcctca gtgttcgctc tgcagctgaa gctgtggcca cctcggtcct cactcagatg
     1381 gccagccaaa ggacagaact gagcatgccg atacaatcgc atgtaatcat gaccccacag
     1441 tcccagtctg cgggcagata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1501 tggctttata tatcttgtgg aaaggacgac agtctgggcc gtcgtgccca gtt
//
