LOCUS       sequence-278858-        1181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0991
FEATURES             Location/Qualifiers
     source          1..1181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtcgatat ggataaactc ataaacaact tggaggtcca acttaattca
       61 gaaggtggct caatgcaggt attcaagcag gtcactgctt ctgttcggaa cagagatccc
      121 cctgagatag aatacagaag taatatgact tctccaacac tcctggatgc caaccccatg
      181 gagaacccag cactgtttaa tgacatcaag attgagcccc cagaagaact tttggctagt
      241 gatttcagcc tgccccaagt ggaaccagtt gacctctcct ttcacaagcc caaggctcct
      301 ctccagcctg ctagcatgct acaagctcca atacgtcccc ccaagccaca gtcttctccc
      361 cagacccttg tggtgtccac gtcaacatct gacatgagca cttcagcaaa cattcctact
      421 gttctgaccc caggctctgt cctgacctcc tctcagagca ctggtagcca gcagatctta
      481 catgtcattc acactatccc ctcagtcagt ctgccaaata agatgggtgg cctgaagacc
      541 atcccagtgg tagtgcagtc tctgcccatg gtgtatacta ctttgcctgc agatgggggc
      601 cctgcagcca ttacagtccc actcattgga ggagatggta aaaatgctgg atcagtgaaa
      661 gttgacccca cctccatgtc tccactggaa attccaagtg acagtgagga gagtacaatt
      721 gagagtggat cctcagcctt gcagagtctg cagggactac agcaagaacc agcagcaatg
      781 gcccaaatgc agggagaaga gtcgcttgac ttgaagagaa gacggattca ccaatgtgac
      841 tttgcaggat gcagcaaagt gtacaccaaa agctctcacc tgaaagctca ccgcagaatc
      901 catacaggag agaagcctta taaatgcacc tgggatggct gctcctggaa atttgctcgc
      961 tcagatgagc tcactcgcca tttccgcaag cacacaggca tcaagccttt tcggtgcaca
     1021 gactgcaacc gcagcttttc tcgttctgac cacctgtccc tgcatcgccg tcgccatgac
     1081 accatgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1141 tcttgtggaa aggacgacgc gggttaattc cctccgttcc c
//
