LOCUS       sequence-278912-        2162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0392
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtggt aacatttggc gatgtggctg tgcacttctc tcgggaggag
       61 tggcagtgtc tggaccctgg ccagagggcc ctctacaggg aagtgatgct ggagaaccac
      121 agcagtgtgg ctggactagc aggattcctg gttttcaagc ctgagctgat ctctcggctg
      181 gagcagggag aagagccatg ggtcctcgac ctgcagggag cagaggggac agaggcacca
      241 aggacctcca agacagattc tacgattagg actgaaaatg agcaggcctg tgaggacatg
      301 gacatcctaa aatcagaatc ctatgggaca gtggtcagaa tctccccaca ggactttcct
      361 cagaatcctg gctttggaga cgtttctgat tctgaggtct ggttagacag tcatctgggc
      421 agtcccgggc tgaaagtgac aggctttacc ttccaaaata actgtttgaa tgaggagact
      481 gtggttccca agaccttcac caaggacgca ccccagggat gtaaggagct gggaagcagc
      541 ggcctggatt gtcagcctct tgaaagtcag ggagagagtg cggaagggat gtcccagaga
      601 tgcgaggagt gtggcaaagg catcagagcc acttcagata tcgctctgca ttgggaaatt
      661 aatacacaga aaattagcag atgtcaagaa tgccaaaaaa agttatctga ctgcttgcag
      721 gggaaacata caaataactg ccatggagag aagccgtacg aatgtgcaga gtgtgggaaa
      781 gtcttcaggc tctgctcgca gcttaatcag catcagagaa tccacacggg agagaaaccc
      841 tttaaatgca ctgagtgtgg aaaagccttc cgcctgagct caaaacttat tcagcatcaa
      901 agaatccaca ctggggagaa gccctacaga tgtgaggaat gtggaaaagc ttttggtcag
      961 agctcaagcc tcatccacca tcagagaatc cacacaggag agaggcccta tggttgtcgt
     1021 gagtgtggga aagccttcag ccagcagtcg cagctggtta gacaccagag aactcacact
     1081 ggggagaggc cctacccttg caaggagtgt gggaaggcct tcagccagag ctccacccta
     1141 gcccagcatc aaaggatgca tactggggag aaagctcaaa ttctaaaagc ctcagacagt
     1201 ccaagccttg ttgcacatca gagaattcac gctgtagaga aaccatttaa gtgtgatgag
     1261 tgtgggaaag cttttaggtg gatctctcgc ctgagtcagc atcagctgat tcacactgga
     1321 gagaagcctt ataaatgcaa caagtgtaca aaagcctttg gttgtagttc acggcttatt
     1381 cgccatcaga gaactcacac tggagaaaaa ccatttaaat gtgatgagtg tggcaaaggc
     1441 tttgttcagg gctcacacct tattcagcat cagcgaatcc acactggaga gaaaccctat
     1501 gtgtgtaatg actgtggaaa agccttcagt cagagttcca gccttattta ccatcagaga
     1561 atccataaag gagagaagcc ctacgaatgc ctccaatgcg gaaaagcctt cagtatgagc
     1621 acacagctta caatacatca aagggttcac actggagaga ggccctataa atgtaatgaa
     1681 tgtgggaaag ccttcagtca aaactcaacc cttttccaac accagataat tcatgcaggg
     1741 gtgaagccct atgagtgcag tgagtgtgga aaagccttca gccggagctc atatcttatt
     1801 gaacaccaga gaatacacac tagggcccag tggttttacg aatatgggaa tgccctggaa
     1861 gggtccacct ttgtgagccg taaaaaggtt aatactataa agaaactgca tcagtgtgaa
     1921 gactgtgaga agatatttag gtggcgttca cacctaatta tacaccagag aattcacacc
     1981 ggggagaagc cttataaatg caatgactgt ggcaaagctt ttaatcgtag ctcaaggctt
     2041 acccagcatc aaaaaattca catgggatag taatgaacta gtccgtaact tgaaagtatt
     2101 tcgatttctt ggctttatat atcttgtgga aaggacgaaa ttgtgggcgc aaaccacagc
     2161 ac
//
