LOCUS       sequence-278918-        2096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0419
FEATURES             Location/Qualifiers
     source          1..2096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagatga tggcagcccc ccagcgaaaa aaatgataac ggacattcat
       61 gcaaatggaa aaacgataaa caaggtgcca acagttaaga aggaacactt ggatgactat
      121 ggagaagcac cagtggaaac tgatggagag catgttaagc gaacctgtac ttctgttcct
      181 gaaactttgc atttaaatcc cagtttgaaa cacacattgg cacaattcca tttaagtagt
      241 cagagctcgc tgggtggacc agcagcattt tctgctcggc attcccaaga aagcatgtcg
      301 cctactgtat ttctgcctct tccatcacct caggttcttc ctggcccatt gctcatccct
      361 tcagatagct ccacagaact cactcagact gtgttggaag gggaatctat ttcttgtttt
      421 caagttggag gagaaaagag actctgtttg ccccaagtct taaattctgt tctccgagaa
      481 tttacactcc agcaaataaa tacagtgtgt gatgaactgt acatatattg ttcaaggtgt
      541 acttcagacc agcttcatat cttaaaggta ctgggcatac ttccattcaa tgccccatcc
      601 tgtgggctga ttacattaac tgatgcacaa agattatgta atgctttatt gcggccacga
      661 acttttcctc aaaatggtag cgtacttcct gctaaaagct cattggccca gttaaaggaa
      721 actggcagtg cctttgaagt ggagcatgaa tgcctaggca aatgtcaggg tttatttgca
      781 ccccagtttt atgttcagcc tgatgctccg tgtattcaat gtctggagtg ttgtggaatg
      841 tttgcacccc agacgtttgt gatgcattct cacagatcac ctgacaaaag aacttgccac
      901 tggggctttg aatcagctaa atggcattgc tatcttcatg tgaaccaaaa atacttagga
      961 acacctgaag aaaagaaact gaagataatt ttagaagaaa tgaaggagaa gtttagcatg
     1021 agaagtggaa agagaaatca atccaagaca gatgcaccat caggaatgga attacagtca
     1081 tggtatcctg ttataaagca ggaaggtgac catgtttctc agacacattc atttttacac
     1141 cccagctact acttatacat gtgtgataaa gtggttgccc caaatgtgtc acttacttct
     1201 gctgtatccc agtctaaaga gctcacaaag acagaggcaa gtaagtccat atcaagacag
     1261 tcagagaagg ctcacagtag tggtaaactt caaaaaacag tgtcttatcc agatgtctca
     1321 cttgaggaac aggagaaaat ggatttaaaa acaagtagag aattatgtag ccgtttagat
     1381 gcatcaatct caaataattc tacaagtaaa aggaaatctg agtctgccac ttgcaactta
     1441 gtcagagaca taaacaaagt gggaattggc cttgttgctg ccgcttcatc tccgcttctt
     1501 gtgaaagatg tcatttgtga ggatgataag ggaaaaatca tggaagaagt aatgagaact
     1561 tatttaaaac aacaggaaaa actaaacttg attttgcaaa agaagcaaca acttcagatg
     1621 gaagtaaaaa tgttgagtag ttcaaaatct atgaaggaac tcactgaaga acagcagaat
     1681 ttacagaaag agcttgaatc tttgcagaat gaacatgctc aaagaatgga agaattttat
     1741 gttgaacaga aagacttaga gaaaaaattg gagcagataa tgaagcaaaa atgtacctgt
     1801 gactcaaatt tagaaaaaga caaagaggct gaatatgcag gacagttggc agaactgagg
     1861 cagagattgg accatgctga ggccgatagg caagaactcc aagatgaact cagacaggaa
     1921 cgggaagcaa gacagaagtt agagatgatg ataaaagagc taaagctgca aattctgaaa
     1981 tcatcaaaga ctgctaaaga atagtaatga actagtccgt aacttgaaag tatttcgatt
     2041 tcttggcttt atatatcttg tggaaaggac gataagggag ccatttgaac gcttat
//
