LOCUS       sequence-278927-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0425
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgatca atccaggatg ctgcagactc tggccggggt gaacctggct
       61 ggccactcgg tgcagggggg catggccctg ccgcctcccc cgcacggcca cgaaggggcg
      121 gacggcgacg gcaggaagca ggacatcggc gacatcctcc accagatcat gaccatcacc
      181 gaccagagct tggacgaggc gcaagcaaag aaacatgccc tgaactgtca cagaatgaaa
      241 ccagcgctct tcagcgtcct gtgtgagatc aaagagaaaa caggtctcag catcagagga
      301 gcccaggagg aggaccctcc cgatccccag ctaatgagac tggacaatat gcttttggca
      361 gaaggggttt caggtcctga gaaaggtggg ggatcggcgg cagcagctgc agccgcggca
      421 gcctctggag gttcttcaga taactctatt gaacactcag attacagagc caaattgacc
      481 cagatcagac aaatctatca cacagaactg gagaaatatg aacaggcatg taatgaattt
      541 actacacatg tgatgaacct tctccgagaa cagagtagaa cacgtcccat ttctccaaaa
      601 gagattgaaa gaatggtggg catcatccat cgaaaattta gttccattca gatgcagctc
      661 aaacaaagca cttgtgaagc agttatgatt ttaagatcaa ggttccttga tgccagacgg
      721 aaaaggcgta acttcagtaa acaggccaca gaaatcttga atgaatattt ttactcacac
      781 ctcagcaacc cctaccccag tgaagaagcc aaagaggagc tggccaagaa atgcagcatc
      841 acagtgtcac aggtatccaa ttggtttggc aacaaacgaa tcaggtacaa gaagaacatt
      901 ggcaagtttc aggaagaagc caacctctat gctgcaaaga cggccgtgac agctgcacac
      961 gcagtagcag cagctgtgca gaacaaccag accaattcgc ccaccacacc aaattccggt
     1021 ggataccctc cgtcatgtta tcaatcagac gggaggctac agtagtaatg aactagtccg
     1081 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagtggtaa
     1141 cgccaaatat gaagcct
//
