LOCUS       sequence-278951-        1457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0478
FEATURES             Location/Qualifiers
     source          1..1457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaaagct ctacgaggca gtgaccttca aggatgtggc tgtgatcttc
       61 actgaggagg agctggggct gctggaccct gcccagagga agctgtaccg agatgtgatg
      121 cttgagaact tcaggaacct gctctcagtg ggaggcaaga tccaaactga gatggagact
      181 gttccagaag caggaacaca tgaagaattt tcctgcaagc aaatttggga acaaattgca
      241 agtgacttaa ccaggtctca agataccacc ataagtaact ctcagttatt tgaacaagat
      301 gacaacccct cccagattaa agcaagacta tctacagttc acacaagaga aaaacctttc
      361 cagggtgaaa attgtaaaca gttcttcagt gatgtttcct tctttgatct tcctcagcag
      421 ttatattcag gagagaagtc tcatacctgt gatgagtgtg gaaaaagctt ctgttacatc
      481 tcagcccttc atattcatca gagggtccac atgggagtga aatgctataa gtgtgatgtg
      541 tgtggtaagg aatttagtca gagctcacgt ctgcaaactc atcaaagagt ccacactgga
      601 gagaaaccat tcaaatgtga gcagtgtggg aaaggcttca gatgtagatc agcacttaaa
      661 gttcattgca aattacacat gagagagaaa ccttataatt gtgagaaatg tgggaaggct
      721 ttcatgcaca atttccagct tcagaaacat cacagaattc atactgggga gaagccattc
      781 aaatgtgaaa tatgtggtaa gagcttctgt cttaggtcaa gtcttaatag gcattgcatg
      841 gtccacacag cagagaaact gtacaaatct gaaaagtatg gaagaggttt cattgatagg
      901 ctagatttgc ataagcatca gatgattcat atgggacaga aaccatataa ttgtaaagaa
      961 tgtgggaaga gcttcaaatg gtcctcatat cttttggtcc atcaacgagt ccacactgga
     1021 gaaaagccat acaaatgtga ggagtgtggg aagggctaca ttagtaagtc aggtcttgac
     1081 ttccaccata gaacccacac gggagagaga tcttataact gtgataactg cgggaagagc
     1141 tttagacatg cttctagtat tttgaatcat aagaaactcc actgccaaag aaagccattg
     1201 aaatgtgaag actgtggaaa gaggcttgta tgccggtcat actgtaaaga ccaacaaaga
     1261 gaccacagtg gagaaaaccc atccaaatgt gaggactgtg ggaagcgcta caagaggcgc
     1321 ttgaatctgg atataatttt atcattattt ttaaatgaca tatagtaatg aactagtccg
     1381 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagtcaacg
     1441 gacatcgagt gatattg
//
