LOCUS       sequence-278960-        1871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0501
FEATURES             Location/Qualifiers
     source          1..1871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagtggc ccggggagtc ggaagcccgg agccagcgcc gccgcagcta
       61 tataagtggg ggggctgtgg gctgggggag cccggcagcg ctttggagag gcgaggagcc
      121 gccgcccgag gccggtgcgg gcgagcgagg gcgccgcggc tccccgactc ctttcccaga
      181 ggtgagtgcc cgaagccagg agcccgggcg cctaggtctg tgcgctgcgg ggaaccccta
      241 ccgccagcct ccccgccacc cgcgcgcccc caagcccagc gggcgaggcc ccgggcgccc
      301 cacagccggc gccgcgccat gctccacctt agcgagtttt ccgaacccga cgcgctcctc
      361 gtcaagtcca ctgaaggctg ttgcgccgaa cccagcgctg aattgccccg gctgcctgcc
      421 agggacgctc ccgcggccac cggctaccct ggagcaggcg acttcttgag ctgggctttg
      481 aacagctgcg gcgcaagtgg ggacttagcc gactcctgct tcctggaggg gcctgcgccc
      541 acaccccctc ccggcctcag ctacagcggt agcttcttca ttcaggcagt gcccgaacac
      601 ccgcacgacc cggaggcact cttcaacctc atgtcgggca tcttaggcct ggcacccttc
      661 cccggtccag aggcagcagc gtccagatcc ccgctggatg ccccttttcc tgcggggtcc
      721 gatgccttgc tgccgggtcc gccggacctt tactccccgg atctgggcgc tgcccctttc
      781 ccagaggcgt tctgggaggc ctcgccttgc gcgggtgccc cctcgcagtg cctgtatgag
      841 cctcagctct ccccgcccga cgtcaagccc ggcctccggg cgcctcccgc ctcgccagcg
      901 ctggacgctg tctctgcctt caagggtccc tacgcgccct gggagctgct ttctgtgggg
      961 gccccaggga actgtgggtc acagggagac taccaggccg ccccggaggc tcgttttccc
     1021 gtaataggga ccaagattga ggacttgctg tccatcagct gccctgcgga actgccggcc
     1081 gtcccagcca acagactcta tcccagcggg gcctatgacg ctttcccgct ggccccgggt
     1141 gacttagggg agggggctga gggcctccct gggctcctga cccctcctag tggggaggga
     1201 gggagtagcg gcgacggcgg agagtttctg gccagtacgc agcctcagct ttccccgctg
     1261 ggccttcgca gcgccgccgc ggcggacttc cctaaacctc tggtggcgga catccctgga
     1321 agcagtggcg tggctgcacc acccgtgccg ccgccgccgc ccaccccttt cccccaggcc
     1381 aaggcgcgac gcaaggggcg ccgcggcggc aaatgcagca cgcgctgctt ctgcccgcgg
     1441 ccgcacgcca aggccttcgc ttgcccggtg gagagttgtg tgcggagctt tgcgcgctcc
     1501 gacgagctca atcgccacct gcgcatccac acgggccaca aacccttcca gtgccgcatc
     1561 tgcctccgca acttcagccg cagcgaccac ctcaccacgc acgtgcgcac ccacaccggc
     1621 gagaagcctt ttgcttgcga cgtgtgcggc cgccgcttcg cgcgcagcga tgagaagaaa
     1681 cggcacagca aggtgcacct caagcagaag gcgcgcgccg aggagcggct caagggcctc
     1741 ggcttttact cgctgggcct ctccttcgct tctctctagt aatgaactag tccgtaactt
     1801 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagga cacgcatgaa
     1861 tccatggaca a
//
